Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/7762
Type: | Journal article |
Title: | The brn-2 gene regulates the melanocytic phenotype and tumorigenic potential of human melanoma cells |
Author: | Angus, J. Thompson, F. Murphy, K. Baker, E. Sutherland, G. Parsons, P. Sturm, R. |
Citation: | Oncogene, 1995; 11(4):691-700 |
Publisher: | Macmillan Press |
Issue Date: | 1995 |
ISSN: | 0950-9232 1476-5594 |
Abstract: | The Oct transcription factors N-Oct-3 and N-Oct-5 are differentially expressed in normal melanocytes, melanoma tumors and cell lines. We have cloned the human brn-2 gene and have shown that it encodes both the N-Oct-3 and N-Oct-5 octamer binding activities detected in melanoma cells. The brn-2 genomic locus has been mapped to chromosome 6q16 and although chromosomal aberrations are common in this region in melanoma, no deletion or rearrangement of the brn-2 gene in melanoma cell lines was observed. Sequencing of the entire gene showed that there are no intervening sequences within the open reading frame. Antisense RNA-mediated inhibition of brn-2 gene expression in melanoma cells was associated with a change in morphology and loss of melanocytic and neural crest markers, including the melanocyte transcription factor microphthalmia and the TYRP pigmentation genes. In addition, loss of brn-2 in these cells resulted in the complete loss of ability to form tumors in SCID and nu/nu mice. These results suggest roles for brn-2 in the determination of the melanocytic lineage and in the tumorigenic phenotype of melanoma. |
Keywords: | Cell Line Tumor Cells, Cultured Chromosomes, Human, Pair 6 Animals Humans Mice Mice, SCID Melanoma Chromosome Deletion Homeodomain Proteins Intercellular Adhesion Molecule-1 Transcription Factors RNA, Antisense DNA, Neoplasm Blotting, Southern In Situ Hybridization, Fluorescence Restriction Mapping Transfection Cell Differentiation Gene Expression Gene Expression Regulation, Neoplastic Base Sequence Molecular Sequence Data POU Domain Factors |
Appears in Collections: | Aurora harvest 4 Paediatrics publications |
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