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https://hdl.handle.net/2440/78386
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dc.contributor.author | Gustafsson, O. | - |
dc.contributor.author | Eddes, J. | - |
dc.contributor.author | Meding, S. | - |
dc.contributor.author | McColl, S. | - |
dc.contributor.author | Oehler, M. | - |
dc.contributor.author | Hoffmann, P. | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | Rapid Communications in Mass Spectrometry, 2013; 27(6):655-670 | - |
dc.identifier.issn | 0951-4198 | - |
dc.identifier.issn | 1097-0231 | - |
dc.identifier.uri | http://hdl.handle.net/2440/78386 | - |
dc.description.abstract | <h4>Rationale</h4>Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry provides the means to map the in situ distribution of tryptic peptides in formalin-fixed clinical tissue samples. The ability to analyze clinical samples is of great importance to further developments in the imaging field. However, there is a requirement in this field of research for additional methods describing the characterization of tryptic peptides by MALDI imaging.<h4>Methods and results</h4>This protocol gives highly detailed instructions, with examples, for (1) successfully performing tryptic peptide MALDI imaging on formalin-fixed cancer tissue using a MALDI-TOF/TOF MS instrument, (2) tentatively generating identifications through nLC/MS/MS, and (3) validating these identifications by in situ MS/MS of peptides of interest.<h4>Conclusions</h4>This protocol provides a detailed and straightforward description of the methods required for groups new to MALDI imaging to begin analysis of formalin-fixed clinical samples. | - |
dc.description.statementofresponsibility | Ove J. R. Gustafsson, James S. Eddes, Stephan Meding, Shaun R. McColl, Martin K. Oehler and Peter Hoffmann | - |
dc.language.iso | en | - |
dc.publisher | John Wiley & Sons Ltd | - |
dc.rights | Copyright © 2013 John Wiley & Sons, Ltd. | - |
dc.source.uri | http://dx.doi.org/10.1002/rcm.6488 | - |
dc.subject | Humans | - |
dc.subject | Neoplasms | - |
dc.subject | Formaldehyde | - |
dc.subject | Trypsin | - |
dc.subject | Peptide Fragments | - |
dc.subject | Calibration | - |
dc.subject | Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | - |
dc.subject | Histocytological Preparation Techniques | - |
dc.subject | Amino Acid Sequence | - |
dc.subject | Image Processing, Computer-Assisted | - |
dc.subject | Molecular Sequence Data | - |
dc.subject | Tandem Mass Spectrometry | - |
dc.subject | Molecular Imaging | - |
dc.title | Matrix-assisted laser desorption/ionization imaging protocol for in situ characterization of tryptic peptide identity and distribution in formalin-fixed tissue | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1002/rcm.6488 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/LP110100693 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/LP110100693 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | McColl, S. [0000-0003-0949-4660] | - |
dc.identifier.orcid | Oehler, M. [0000-0002-2651-5913] | - |
Appears in Collections: | Aurora harvest IPAS publications |
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