Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/7851
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Evidence for a functional Hepatocyte Growth Factor receptor in human mesangial cells |
Author: | Kallincos, N. Pollard, A. Couper, J. |
Citation: | Regulatory Peptides, 1998; 74(2-3):137-142 |
Publisher: | Elsevier Science |
Issue Date: | 1998 |
ISSN: | 0167-0115 1873-1686 |
Statement of Responsibility: | Nicholas C Kallincos, Angie N Pollard, Jennifer J Couper |
Abstract: | In this study we have investigated both the expression of c-met in cultured human mesangial cells and the proliferative effect of HGF on these cells. RNAse protection analysis using a c-met riboprobe showed c-met to be expressed and further that this expression was unaffected by the glucose concentration or osmolality of the media. Immunofluorescence studies performed using anti-HGF or anti-c-met antibodies clearly showed that both proteins are localised to human mesangial cells. Proliferation of human mesangial cells after 24-h treatment with HGF was also examined. HGF 10 ng/ml and 100 ng/ml stimulated 3-H-Thymidine incorporation 1.35-fold (P=0.001) and 1.6-fold (P<0.00001) respectively in cells made quiescent for 24 h. A similar dose-dependent stimulation of proliferation was observed in cells made quiescent for 48 h. Finally, using RNAse protection analysis we have shown that HGF (10 ng/ml, 100 ng/ml) induces the expression of c-met in these cells in a dose-dependant manner. Together these results indicate for the first time a potential autocrine role for HGF in the human mesangium. |
Keywords: | c-met; Gene expression; Proliferation; Autocrine actions |
Rights: | © 1998 Elsevier Science B.V. |
DOI: | 10.1016/S0167-0115(98)00034-2 |
Published version: | http://dx.doi.org/10.1016/s0167-0115(98)00034-2 |
Appears in Collections: | Aurora harvest 4 Paediatrics publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.