Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/79036
Citations | ||
Scopus | Web of ScienceĀ® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | A radish seed antifungal peptide with a high amyloid fibril-forming propensity |
Author: | Garvey, M. Meehan, S. Gras, S. Schirra, H. Craik, D. Van der Weerden, N. Anderson, M. Gerrard, J. Carver, J. |
Citation: | BBA: Proteins and Proteomics, 2013; 1834(8):1615-1623 |
Publisher: | Elsevier Science BV |
Issue Date: | 2013 |
ISSN: | 1570-9639 1878-1454 |
Organisation: | Institute for Photonics & Advanced Sensing (IPAS) |
Statement of Responsibility: | Megan Garvey, Sarah Meehan, Sally L. Gras, Horst J. Schirra, David J. Craik, Nicole L. Van der Weerden, Marilyn A. Anderson, Juliet A. Gerrard, John A. Carver |
Abstract: | The amyloid fibril-forming ability of two closely related antifungal and antimicrobial peptides derived from plant defensin proteins has been investigated. As assessed by sequence analysis, thioflavin T binding, transmission electron microscopy, atomic force microscopy and X-ray fiber diffraction, a 19 amino acid fragment from the C-terminal region of Raphanus sativus antifungal protein, known as RsAFP-19, is highly amyloidogenic. Further, its fibrillar morphology can be altered by externally controlled conditions. Freezing and thawing led to amyloid fibril formation which was accompanied by loss of RsAFP-19 antifungal activity. A second, closely related antifungal peptide displayed no fibril-forming capacity. It is concluded that while fibril formation is not associated with the antifungal properties of these peptides, the peptide RsAFP-19 is of potential use as a controllable, highly amyloidogenic small peptide for investigating the structure of amyloid fibrils and their mechanism of formation. |
Keywords: | Amyloid fibril Antifungal peptide Antimicrobial peptide Protein misfolding Protein aggregation |
DOI: | 10.1016/j.bbapap.2013.04.030 |
Grant ID: | ARC |
Published version: | http://dx.doi.org/10.1016/j.bbapap.2013.04.030 |
Appears in Collections: | Aurora harvest IPAS publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.