Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/79639
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Prostaglandin ethanolamides attenuate damage in a human explant colitis model |
Author: | Nicotra, L. Vu, M. Harvey, B. Smid, S. |
Citation: | Prostaglandins and Other Lipid Mediators, 2013; 100(1):22-29 |
Publisher: | Elsevier Inc. |
Issue Date: | 2013 |
ISSN: | 1098-8823 2212-196X |
Statement of Responsibility: | Lauren L. Nicotra, Megan Vu, Benjamin S. Harvey and Scott D. Smid |
Abstract: | Endocannabinoids are protective in animal colitis models. As endocannabinoids also form novel prostaglandin ethanolamides (prostamides) via COX-2, we investigated the effects of prostamides and other COX-2 mediators on tissue damage in an ex vivo human mucosal explant colitis model. Healthy human colonic mucosae were incubated with pro-inflammatory cytokines TNF-α and IL-1β to elicit colitis-like tissue damage. The PGF-ethanolamide analogue, bimatoprost decreased colitis scores which were reversed by a prostamide-specific antagonist AGN 211334, but not the FP receptor antagonist AL-8810. PGF-ethanolamide and PGE-ethanolamide also reduced cytokine-evoked epithelial damage. Anandamide was protective in the explant colitis model; however COX-2 inhibition did not alter its effects, associated with a lack of COX-2 induction in explant mucosal tissue. These findings support an anti-inflammatory role for prostamides and endocannabinoids in the human colon. |
Keywords: | Colon, Sigmoid Humans Colitis Amides Sulfonamides Oxazoles Arachidonic Acids Dinoprostone Dinoprost Cloprostenol Tumor Necrosis Factor-alpha Receptors, Prostaglandin Cyclooxygenase Inhibitors Endocannabinoids Tissue Culture Techniques Immunohistochemistry Adult Middle Aged Female Male Cyclooxygenase 2 Polyunsaturated Alkamides Interleukin-1beta Young Adult Bimatoprost |
Rights: | © 2013 Elsevier Inc. All rights reserved. |
DOI: | 10.1016/j.prostaglandins.2013.01.003 |
Published version: | http://dx.doi.org/10.1016/j.prostaglandins.2013.01.003 |
Appears in Collections: | Aurora harvest Pharmacology publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.