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https://hdl.handle.net/2440/79673
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Type: | Journal article |
Title: | Potential functional replacement of the plastidic acetyl-CoA carboxylase subunit (accD) gene by recent transfers to the nucleus in some angiosperm lineages |
Author: | Rousseau, M. Huang, X. Higginson, E. Ayliffe, M. Day, A. Timmis, J. |
Citation: | Plant Physiology, 2013; 161(4):1918-1929 |
Publisher: | Amer Soc Plant Physiologists |
Issue Date: | 2013 |
ISSN: | 0032-0889 1532-2548 |
Statement of Responsibility: | Mathieu Rousseau-Gueutin, Xun Huang, Emily Higginson, Michael Ayliffe, Anil Day and Jeremy N. Timmis |
Abstract: | Eukaryotic cells originated when an ancestor of the nucleated cell engulfed bacterial endosymbionts that gradually evolved into the mitochondrion and the chloroplast. Soon after these endosymbiotic events, thousands of ancestral prokaryotic genes were functionally transferred from the endosymbionts to the nucleus. This process of functional gene relocation, now rare in eukaryotes, continues in angiosperms. In this article, we show that the chloroplastic acetyl-CoA carboxylase subunit (accD) gene that is present in the plastome of most angiosperms has been functionally relocated to the nucleus in the Campanulaceae. Surprisingly, the nucleus-encoded accD transcript is considerably smaller than the plastidic version, consisting of little more than the carboxylase domain of the plastidic accD gene fused to a coding region encoding a plastid targeting peptide. We verified experimentally the presence of a chloroplastic transit peptide by showing that the product of the nuclear accD fused to green fluorescent protein was imported in the chloroplasts. The nuclear gene regulatory elements that enabled the erstwhile plastidic gene to become functional in the nuclear genome were identified, and the evolution of the intronic and exonic sequences in the nucleus is described. Relocation and truncation of the accD gene is a remarkable example of the processes underpinning endosymbiotic evolution. |
Keywords: | Cell Nucleus Plastids Campanulaceae Acetyl-CoA Carboxylase Protein Subunits RNA, Messenger Sequence Alignment Gene Transfer, Horizontal Amino Acid Sequence Regulatory Sequences, Nucleic Acid Genes, Plant Introns Molecular Sequence Data Magnoliopsida |
Rights: | © 2013 American Society of Plant Biologists. All Rights Reserved. |
DOI: | 10.1104/pp.113.214528 |
Grant ID: | http://purl.org/au-research/grants/arc/DP0986973 http://purl.org/au-research/grants/arc/DP0986973 |
Published version: | http://dx.doi.org/10.1104/pp.113.214528 |
Appears in Collections: | Aurora harvest Molecular and Biomedical Science publications |
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