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https://hdl.handle.net/2440/79891
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Type: | Journal article |
Title: | Effects of Osteochondrin S and select connective tissue ribonucleinate components on human osteoclasts in vitro |
Author: | Cantley, M. Rainsford, K. Haynes, D. |
Citation: | Journal of Pharmacy and Pharmacology, 2013; 65(8):1214-1222 |
Publisher: | Royal Pharmaceutical Soc Great Britain |
Issue Date: | 2013 |
ISSN: | 0022-3573 2042-7158 |
Statement of Responsibility: | Melissa D. Cantley, K. D. Rainsford and David R. Haynes |
Abstract: | OBJECTIVES Osteochondrin S, a natural product derived from connective tissues and yeast, is used to treat osteoarthritis. The aim of this study was to determine the effect of Osteochondrin S on human osteoclast activity in vitro. METHODS Osteoclasts were derived from human peripheral blood mononuclear cells stimulated with macrophage colony-stimulating factor and receptor activator of nuclear factor kappa B (RANK) ligand. Cells were treated with 23.5–587.2 ng/ml Osteochondrin S or 0.2–5 mg/ml of RNA components (synovia, placenta, intervertebral disc or cartilage). The effects on osteoclast formation and resorptive activity were assessed. Real-time polymerase chain reaction was conducted to assess the expression of key osteoclast genes. KEY FINDINGS Osteochondrin S and the individual RNA extracts resulted in a concentration-dependent inhibition of human osteoclast activity. Osteochondrin S did not affect RANK, nuclear factor of activated T cells (NFATc1), osteoclast-associated receptor or cathepsin K expression. However, there was a significant (P < 0.05) reduction in mRNA expression of calcitonin receptor. Osteochondrin S treatment also significantly increased the expression of osteoclast inhibitory factor interferon-β and, interestingly, increased the expression of tumour necrosis-α-like weak inducer of apoptosis (TWEAK). CONCLUSIONS Osteochondrin S inhibited the resorptive ability of osteoclasts. These actions are likely to occur at a late stage during osteoclast formation, downstream of NFATc1. Overall, the findings show that Osteochondrin S inhibition of osteoclast activity may be responsible for its beneficial effects on diseases such as osteoarthritis. |
Keywords: | Bone resorption in-vitro osteoclast assay Osteochondrin S RNA extracts |
Rights: | © 2013 Royal Pharmaceutical Society, Journal of Pharmacy and Pharmacology |
DOI: | 10.1111/jphp.12088 |
Published version: | http://dx.doi.org/10.1111/jphp.12088 |
Appears in Collections: | Anatomical Sciences publications Aurora harvest 4 |
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