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https://hdl.handle.net/2440/79898
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dc.contributor.author | Wikstrom, M. | - |
dc.contributor.author | Fleming, P. | - |
dc.contributor.author | Comerford, I. | - |
dc.contributor.author | McColl, S. | - |
dc.contributor.author | Andoniou, C. | - |
dc.contributor.author | Degli-Esposti, M. | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | Journal of Virology, 2013; 87(14):7911-7920 | - |
dc.identifier.issn | 0022-538X | - |
dc.identifier.issn | 1098-5514 | - |
dc.identifier.uri | http://hdl.handle.net/2440/79898 | - |
dc.description.abstract | Murine cytomegalovirus encodes numerous proteins that act on a variety of pathways to modulate the innate and adaptive immune responses. Here, we demonstrate that a chemokine-like protein encoded by murine cytomegalovirus activates the early innate immune response and delays adaptive immunity, thereby impairing viral clearance. The protein, m131/129 (also known as MCK-2), is not required to establish infection in the spleen; however, a mutant virus lacking m131/129 was cleared more rapidly from this organ. In the absence of m131/129 expression, there was enhanced activation of dendritic cells (DC), and virus-specific CD8+ T cells were recruited into the immune response earlier. Viral mutants lacking m131/129 elicited weaker production of alpha interferon (IFN-α) at 40 h postinfection, indicating that this protein exerts its effects during early rounds of viral replication in the spleen. Furthermore, while wild-type and mutant viruses activated plasmacytoid dendritic cells (pDC) equally at this time, as measured by the upregulation of costimulatory molecules, the presence of m131/129 stimulated more pDC to secrete IFN-α, accounting for the stronger IFN-α response than from the wild-type virus. These data provide evidence for a novel immunomodulatory function of a viral chemokine and expose the multifunctionality of immune evasion proteins. In addition, these results broaden our understanding of the interplay between innate and adaptive immunity. | - |
dc.description.statementofresponsibility | Matthew E. Wikstrom, Peter Fleming, Iain Comerford, Shaun R. McColl, Christopher E. Andoniou, Mariapia A. Degli-Esposti | - |
dc.language.iso | en | - |
dc.publisher | Amer Soc Microbiology | - |
dc.rights | Copyright © 2013, American Society for Microbiology. All Rights Reserved. | - |
dc.source.uri | http://dx.doi.org/10.1128/jvi.00187-13 | - |
dc.subject | Spleen | - |
dc.subject | Dendritic Cells | - |
dc.subject | CD8-Positive T-Lymphocytes | - |
dc.subject | Animals | - |
dc.subject | Mice, Inbred BALB C | - |
dc.subject | Mice | - |
dc.subject | Muromegalovirus | - |
dc.subject | Interferon-alpha | - |
dc.subject | Viral Proteins | - |
dc.subject | Chemokines | - |
dc.subject | Chemokines, CC | - |
dc.subject | Cytokines | - |
dc.subject | Fluorescent Antibody Technique | - |
dc.subject | Enzyme-Linked Immunosorbent Assay | - |
dc.subject | Flow Cytometry | - |
dc.subject | Statistics, Nonparametric | - |
dc.subject | Lymphocyte Activation | - |
dc.subject | Immunity, Innate | - |
dc.subject | Adaptive Immunity | - |
dc.subject | Immune Evasion | - |
dc.title | A chemokine-like viral protein enhances alpha interferon production by plasmacytoid dendritic cells but delays CD8⁺ T cell activation and impairs viral clearance | - |
dc.title.alternative | A chemokine-like viral protein enhances alpha interferon production by plasmacytoid dendritic cells but delays CD8(+) T cell activation and impairs viral clearance | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1128/JVI.00187-13 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | McColl, S. [0000-0003-0949-4660] | - |
Appears in Collections: | Aurora harvest 4 Molecular and Biomedical Science publications |
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