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https://hdl.handle.net/2440/8025
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Type: | Journal article |
Title: | Cytokine secretion by macrophages in the rat testis |
Author: | Kern, S. Robertson, S. Mau, V. Maddocks, S. |
Citation: | Biology of Reproduction, 1995; 53(6):1407-1416 |
Publisher: | Society for the Study of Reproduction |
Issue Date: | 1995 |
ISSN: | 0006-3363 1529-7268 |
Statement of Responsibility: | Stephan Kern, Sarah A. Robertson, Vicki J. Mau, and Simon Maddocks |
Abstract: | The rat testis contains a large population of resident macrophages, the physiological roles of which are yet to be established. To investigate the functional capacity of these cells, we have analyzed the secretion of the cytokines interleukin (IL)-1, IL-6, tumor necrosis factor alpha (TNF alpha), and granulocyte macrophage-colony stimulating factor (GM-CSF) by isolated testicular macrophages (TMs) and, for comparison, by isolated rat peritoneal macrophages (PMs). Cells were cultured for 48 h in serum-free medium alone or with lipopolysaccharide (LPS, 10 micrograms/ml) and/or recombinant interferon-gamma (rIFN gamma, 200 U/ml). Specific bioassays were used to measure cytokines in the media collected from cultures. Basal production of IL-1, TNF alpha, and IL-6 by TMs and PMs were similar, but TMs produced 8-fold greater levels of GM-CSF than did PMs. LPS, alone or in combination with IFN gamma, significantly enhanced the secretion of all cytokines by PMs (340-840% increase). LPS alone had little effect on TM secretion except to reduce GM-CSF levels some 4-fold. The addition of LPS and IFN gamma increased IL-1, IL-6, and TNF alpha levels (200-750% increase) and reduced GM-CSF levels to 45% of basal levels. Treatment of cultures with indomethacin to minimize prostaglandin production enhanced the LPS-induced effects in both cell types. Expression of the mRNA for each cytokine in cultures of testicular and peritoneal macrophages, as well as in intact testis, was confirmed by reverse transcription polymerase chain reaction. These studies indicate that macrophages resident within the rat testis have a novel cytokine secretion profile and an altered responsiveness to inflammatory activators compared with macrophages from the peritoneal cavity. This may be important in physiological processes in the testis and may contribute to the dysfunctional afferent immune activity thought to underlie the immunologically privileged status of the testes. |
Keywords: | Testis Cells, Cultured Macrophages Macrophages, Peritoneal Animals Rats, Inbred Strains Rats Indomethacin Tumor Necrosis Factor-alpha Granulocyte-Macrophage Colony-Stimulating Factor DNA, Complementary RNA, Messenger Interleukin-1 Interleukin-6 Cytokines Culture Media, Serum-Free Gene Expression Base Sequence Molecular Sequence Data Male |
DOI: | 10.1095/biolreprod53.6.1407 |
Published version: | http://dx.doi.org/10.1095/biolreprod53.6.1407 |
Appears in Collections: | Aurora harvest Obstetrics and Gynaecology publications |
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