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Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/81165
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dc.contributor.authorSt-Pierre, F.-
dc.contributor.authorCui, L.-
dc.contributor.authorPriest, D.-
dc.contributor.authorEndy, D.-
dc.contributor.authorDodd, I.-
dc.contributor.authorShearwin, K.-
dc.date.issued2013-
dc.identifier.citationACS Synthetic Biology, 2013; 2(9):537-541-
dc.identifier.issn2161-5063-
dc.identifier.issn2161-5063-
dc.identifier.urihttp://hdl.handle.net/2440/81165-
dc.description.abstractWe describe "clonetegration", a method for integrating DNA into prokaryotic chromosomes that approaches the simplicity of cloning DNA within extrachromosomal vectors. Compared to existing techniques, clonetegration drastically decreases the time and effort needed for integration of single or multiple DNA fragments. Additionally, clonetegration facilitates cloning and expression of genetic elements that are impossible to propagate within typical multicopy plasmids.-
dc.description.statementofresponsibilityFrançois St-Pierre, Lun Cui, David G. Priest, Drew Endy, Ian B. Dodd, and Keith E. Shearwin-
dc.language.isoen-
dc.publisherAmerican Chemical Society-
dc.rightsCopyright © 2013 American Chemical Society-
dc.source.urihttp://dx.doi.org/10.1021/sb400021j-
dc.subjectChromosomes, Bacterial-
dc.subjectEscherichia coli-
dc.subjectSalmonella typhimurium-
dc.subjectDNA-
dc.subjectDNA Primers-
dc.subjectCloning, Molecular-
dc.subjectGenetic Engineering-
dc.subjectTransformation, Genetic-
dc.subjectGenetic Vectors-
dc.titleOne-step cloning and chromosomal integration of DNA-
dc.typeJournal article-
dc.identifier.doi10.1021/sb400021j-
dc.relation.granthttp://purl.org/au-research/grants/arc/DP110101470-
dc.relation.granthttp://purl.org/au-research/grants/arc/DP110100824-
pubs.publication-statusPublished-
dc.identifier.orcidDodd, I. [0000-0003-2969-6841]-
dc.identifier.orcidShearwin, K. [0000-0002-7736-2742]-
Appears in Collections:Aurora harvest 4
Molecular and Biomedical Science publications

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