Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/81209
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Duck hepatitis B virus covalently closed circular DNA appears to survive hepatocyte mitosis in the growing liver |
Author: | Reaiche-Miller, G. Thorpe, M. Low, H. Qiao, Q. Scougall, C. Mason, W. Litwin, S. Jilbert, A. |
Citation: | Virology, 2013; 446(1-2):357-364 |
Publisher: | Academic Press Inc |
Issue Date: | 2013 |
ISSN: | 0042-6822 1096-0341 |
Statement of Responsibility: | Georget Y. Reaiche-Miller, Michael Thorpe, Huey Chi Low, Qiao Qiao, Catherine A. Scougall, William S. Mason, Samuel Litwin, Allison R. Jilbert |
Abstract: | Nucleos(t)ide analogues that inhibit hepatitis B virus (HBV) DNA replication are typically used as monotherapy for chronically infected patients. Treatment with a nucleos(t)ide analogue eliminates most HBV DNA replication intermediates and produces a gradual decline in levels of covalently closed circular DNA (cccDNA), the template for viral RNA synthesis. It remains uncertain if levels of cccDNA decline primarily through hepatocyte death, or if loss also occurs during hepatocyte mitosis. To determine if cccDNA survives mitosis, growing ducklings infected with duck hepatitis B virus (DHBV) were treated with the nucleoside analogue, Entecavir. Viremia was suppressed at least 10(5)-fold, during a period when average liver mass increased 23-fold. Analysis of the data suggested that if cccDNA synthesis was completely inhibited, at least 49% of cccDNA survived hepatocyte mitosis. However, there was a large duck-to-duck variation in cccDNA levels, suggesting that low level cccDNA synthesis may contribute to this apparent survival through mitosis. |
Keywords: | Hepatitis B virus (HBV) Duck hepatitis B virus (DHBV) Covalently closed circular DNA (cccDNA) Hepatocyte mitosis Nucleoside analogue Entecavir (ETV) Survival or loss of cccDNA |
Rights: | Crown copyright © 2013 |
DOI: | 10.1016/j.virol.2013.08.014 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/453506 http://purl.org/au-research/grants/nhmrc/1004847 |
Published version: | http://dx.doi.org/10.1016/j.virol.2013.08.014 |
Appears in Collections: | Aurora harvest 4 Molecular and Biomedical Science publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.