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https://hdl.handle.net/2440/81398
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Type: | Journal article |
Title: | IMGT/HighV QUEST paradigm for T cell receptor IMGT clonotype diversity and next generation repertoire immunoprofiling |
Author: | Li, S. Lefranc, M. Miles, J. Alamyar, E. Giudicelli, V. Duroux, P. Freeman, J. Corbin, V. Scheerlinck, J. Frohman, M. Cameron, P. Plebanski, M. Loveland, B. Burrows, S. Papenfuss, A. Gowans, E. |
Citation: | Nature Communications, 2013; 4(1):1-13 |
Publisher: | Nature Publishing Group |
Issue Date: | 2013 |
ISSN: | 2041-1723 2041-1723 |
Statement of Responsibility: | Shuo Li, Marie-Paule Lefranc, John J. Miles, Eltaf Alamyar, Véronique Giudicelli, Patrice Duroux, J. Douglas Freeman, Vincent D. A. Corbin, Jean-Pierre Scheerlinck, Michael A. Frohman, Paul U. Cameron, Magdalena Plebanski, Bruce Loveland, Scott R. Burrows, Anthony T. Papenfuss, & Eric J. Gowans |
Abstract: | T cell repertoire diversity and clonotype follow-up in vaccination, cancer, infectious and immune diseases represent a major challenge owing to the enormous complexity of the data generated. Here we describe a next generation methodology, which combines 5′RACE PCR, 454 sequencing and, for analysis, IMGT, the international ImMunoGeneTics information system (IMGT), IMGT/HighV-QUEST web portal and IMGT-ONTOLOGY concepts. The approach is validated in a human case study of T cell receptor beta (TRB) repertoire, by chronologically tracking the effects of influenza vaccination on conventional and regulatory T cell subpopulations. The IMGT/HighV-QUEST paradigm defines standards for genotype/haplotype analysis and characterization of IMGT clonotypes for clonal diversity and expression and achieves a degree of resolution for next generation sequencing verifiable by the user at the sequence level, while providing a normalized reference immunoprofile for human TRB. |
Keywords: | Biological sciences Bioinformatics Genetics Immunology |
Rights: | © 2013 Macmillan Publishers Limited. All rights reserved. |
DOI: | 10.1038/ncomms3333 |
Published version: | http://dx.doi.org/10.1038/ncomms3333 |
Appears in Collections: | Aurora harvest Surgery publications |
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