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https://hdl.handle.net/2440/81696
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dc.contributor.author | Liu, Y. | - |
dc.contributor.author | Pukala, T. | - |
dc.contributor.author | Musgrave, I. | - |
dc.contributor.author | Williams, D. | - |
dc.contributor.author | Dehle, F. | - |
dc.contributor.author | Carver, J. | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | Bioorganic and Medicinal Chemistry Letters, 2013; 23(23):6336-6340 | - |
dc.identifier.issn | 0960-894X | - |
dc.identifier.issn | 1464-3405 | - |
dc.identifier.uri | http://hdl.handle.net/2440/81696 | - |
dc.description.abstract | Many protein misfolding diseases, for example, Alzheimer's, Parkinson's and Huntington's, are characterised by the accumulation of protein aggregates in an amyloid fibrillar form. Natural products which inhibit fibril formation are a promising avenue to explore as therapeutics for the treatment of these diseases. In this study we have shown, using in vitro thioflavin T assays and transmission electron microscopy, that grape seed extract inhibits fibril formation of kappa-casein (κ-CN), a milk protein which forms amyloid fibrils spontaneously under physiological conditions. Among the components of grape seed extract, gallic acid was the most active component at inhibiting κ-CN fibril formation, by stabilizing κ-CN to prevent its aggregation. Concomitantly, gallic acid significantly reduced the toxicity of κ-CN to pheochromocytoma12 cells. Furthermore, gallic acid effectively inhibited fibril formation by the amyloid-beta peptide, the putative causative agent in Alzheimer's disease. It is concluded that the gallate moiety has the fibril-inhibitory activity. | - |
dc.description.statementofresponsibility | Yanqin Liu, Tara L. Pukala, Ian F. Musgrave, Danielle M. Williams, Francis C. Dehle, John A. Carver | - |
dc.language.iso | en | - |
dc.publisher | Pergamon-Elsevier Science Ltd | - |
dc.rights | © 2013 Elsevier Ltd. All rights reserved. | - |
dc.source.uri | http://dx.doi.org/10.1016/j.bmcl.2013.09.071 | - |
dc.subject | Amyloid fibril | - |
dc.subject | Grape seed extract | - |
dc.subject | Gallic acid | - |
dc.subject | Kappa-casein | - |
dc.subject | Amyloid-beta peptide | - |
dc.subject | Protein aggregation | - |
dc.title | Gallic acid is the major component of grape seed extract that inhibits amyloid fibril formation | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1016/j.bmcl.2013.09.071 | - |
dc.relation.grant | ARC | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Pukala, T. [0000-0001-7391-1436] | - |
dc.identifier.orcid | Musgrave, I. [0000-0003-1016-0588] | - |
Appears in Collections: | Aurora harvest 4 Chemistry and Physics publications |
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