Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/81931
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Type: | Journal article |
Title: | Pre-maturation with cAMP modulators in conjunction with EGF-like peptides during in vitro maturation enhances mouse oocyte developmental competence |
Author: | Richani, D. Wang, X. Zeng, H. Smitz, J. Thompson, J. Gilchrist, R. |
Citation: | Molecular Reproduction and Development, 2014; 81(5):422-435 |
Publisher: | John Wiley |
Issue Date: | 2014 |
ISSN: | 1098-2795 1098-2795 |
Organisation: | Robinson Institute |
Statement of Responsibility: | D. Richani, X. Wang, H. Zeng, J.E.J. Smitz, J.G. Thompson, and R.B. Gilchrist |
Abstract: | Recent studies have independently shown that cyclic adenosine 3'5'-monophosphate (cAMP) modulation prior to in vitro maturation (IVM) and epidermal growth factor (EGF)-like peptide supplementation during IVM improve subsequent oocyte developmental outcomes. This study investigated the effects of an IVM system that incorporates these two concepts. Cumulus-oocyte complexes (COCs) were collected from pre-pubertal mice either 46 hr post-equine chorionic gonadotropin (eCG) (IVM) or post-eCG + post-human chorionic gonadotropin (hCG) stimulation (in vivo maturation; IVV). IVM COCs were treated with the cAMP modulators forskolin and IBMX for 1, 2, or 4 hr (pre-IVM phase) prior to IVM. COCs then underwent IVM with the EGF-like peptides amphiregulin or epiregulin, or with the common IVM stimulants follicle-stimulating hormone (FSH) or EGF. A pre-IVM phase increased the size of the subsequent blastocysts' inner-cell-mass compared to standard IVM, regardless of IVM treatment (P < 0.05). Unlike FSH or EGF, amphiregulin or epiregulin significantly increased blastocyst quality (trophectoderm and total cell numbers) and/or yield (P < 0.01) compared to standard IVM, and were the only treatments that produced blastocysts comparable to IVV-derived blastocysts. Forskolin acutely up-regulated EGF-like peptide mRNA expression after a 2-hr pre-IVM phase (P < 0.001), although EGF receptor and ERK1/2 activities were not significantly different than control. IVV-like levels of EGF-like peptide mRNA expression during IVM were maintained only by supplementing with EGF-like peptides and EGF, since expression levels induced by FSH were significantly lower in vitro than during IVV. However, EGF receptor and ERK1/2 phosphorylation levels were not significantly different across treatment groups. In conclusion, a pre-IVM phase in conjunction with IVM in the presence of EGF-like peptides endows high oocyte developmental competence, as evidenced by increased embryo yield and/or quality relative to FSH and EGF. |
Keywords: | Amphiregulin epiregulin FSH EGF forskolin IBMX |
Rights: | © 2014 Wiley Periodicals, Inc. |
DOI: | 10.1002/mrd.22307 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1007551 http://purl.org/au-research/grants/nhmrc/1023210 http://purl.org/au-research/grants/nhmrc/627007 |
Published version: | http://dx.doi.org/10.1002/mrd.22307 |
Appears in Collections: | Aurora harvest 4 Obstetrics and Gynaecology publications |
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File | Description | Size | Format | |
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hdl_81931.pdf | Accepted version | 649.22 kB | Adobe PDF | View/Open |
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