Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/81931
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dc.contributor.author | Richani, D. | - |
dc.contributor.author | Wang, X. | - |
dc.contributor.author | Zeng, H. | - |
dc.contributor.author | Smitz, J. | - |
dc.contributor.author | Thompson, J. | - |
dc.contributor.author | Gilchrist, R. | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | Molecular Reproduction and Development, 2014; 81(5):422-435 | - |
dc.identifier.issn | 1098-2795 | - |
dc.identifier.issn | 1098-2795 | - |
dc.identifier.uri | http://hdl.handle.net/2440/81931 | - |
dc.description.abstract | Recent studies have independently shown that cyclic adenosine 3'5'-monophosphate (cAMP) modulation prior to in vitro maturation (IVM) and epidermal growth factor (EGF)-like peptide supplementation during IVM improve subsequent oocyte developmental outcomes. This study investigated the effects of an IVM system that incorporates these two concepts. Cumulus-oocyte complexes (COCs) were collected from pre-pubertal mice either 46 hr post-equine chorionic gonadotropin (eCG) (IVM) or post-eCG + post-human chorionic gonadotropin (hCG) stimulation (in vivo maturation; IVV). IVM COCs were treated with the cAMP modulators forskolin and IBMX for 1, 2, or 4 hr (pre-IVM phase) prior to IVM. COCs then underwent IVM with the EGF-like peptides amphiregulin or epiregulin, or with the common IVM stimulants follicle-stimulating hormone (FSH) or EGF. A pre-IVM phase increased the size of the subsequent blastocysts' inner-cell-mass compared to standard IVM, regardless of IVM treatment (P < 0.05). Unlike FSH or EGF, amphiregulin or epiregulin significantly increased blastocyst quality (trophectoderm and total cell numbers) and/or yield (P < 0.01) compared to standard IVM, and were the only treatments that produced blastocysts comparable to IVV-derived blastocysts. Forskolin acutely up-regulated EGF-like peptide mRNA expression after a 2-hr pre-IVM phase (P < 0.001), although EGF receptor and ERK1/2 activities were not significantly different than control. IVV-like levels of EGF-like peptide mRNA expression during IVM were maintained only by supplementing with EGF-like peptides and EGF, since expression levels induced by FSH were significantly lower in vitro than during IVV. However, EGF receptor and ERK1/2 phosphorylation levels were not significantly different across treatment groups. In conclusion, a pre-IVM phase in conjunction with IVM in the presence of EGF-like peptides endows high oocyte developmental competence, as evidenced by increased embryo yield and/or quality relative to FSH and EGF. | - |
dc.description.statementofresponsibility | D. Richani, X. Wang, H. Zeng, J.E.J. Smitz, J.G. Thompson, and R.B. Gilchrist | - |
dc.language.iso | en | - |
dc.publisher | John Wiley | - |
dc.rights | © 2014 Wiley Periodicals, Inc. | - |
dc.source.uri | http://dx.doi.org/10.1002/mrd.22307 | - |
dc.subject | Amphiregulin | - |
dc.subject | epiregulin | - |
dc.subject | FSH | - |
dc.subject | EGF | - |
dc.subject | forskolin | - |
dc.subject | IBMX | - |
dc.title | Pre-maturation with cAMP modulators in conjunction with EGF-like peptides during in vitro maturation enhances mouse oocyte developmental competence | - |
dc.type | Journal article | - |
dc.contributor.organisation | Robinson Institute | - |
dc.identifier.doi | 10.1002/mrd.22307 | - |
dc.relation.grant | http://purl.org/au-research/grants/nhmrc/1007551 | - |
dc.relation.grant | http://purl.org/au-research/grants/nhmrc/1023210 | - |
dc.relation.grant | http://purl.org/au-research/grants/nhmrc/627007 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Thompson, J. [0000-0003-4941-7731] | - |
dc.identifier.orcid | Gilchrist, R. [0000-0003-1611-7142] | - |
Appears in Collections: | Aurora harvest 4 Obstetrics and Gynaecology publications |
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hdl_81931.pdf | Accepted version | 649.22 kB | Adobe PDF | View/Open |
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