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https://hdl.handle.net/2440/82722
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Type: | Journal article |
Title: | The PSF·p54nrb complex is a novel Mnk substrate that binds the mRNA for tumor necrosis factor α |
Other Titles: | The PSF(.) p54(nrb) complex is a novel Mnk substrate that binds the mRNA for tumor necrosis factor alpha |
Author: | Buxade, M. Morrice, N. Krebs, D. Proud, C. |
Citation: | Journal of Biological Chemistry, 2008; 283(1):57-65 |
Publisher: | Amer Soc Biochemistry Molecular Biology Inc |
Issue Date: | 2008 |
ISSN: | 0021-9258 1083-351X |
Statement of Responsibility: | Maria Buxadé, Nick Morrice, Danielle L. Krebs and Christopher G. Proud |
Abstract: | To identify new potential substrates for the MAP kinase signal-integrating kinases (Mnks), we employed a proteomic approach. The Mnks are targeted to the translational machinery through their interaction with the cap-binding initiation factor complex. We tested whether proteins retained on cap resin were substrates for the Mnks in vitro, and identified one such protein as PSF (the PTB (polypyrimidine tract-binding protein)-associated splicing factor). Mnks phosphorylate PSF at two sites in vitro, and our data show that PSF is an Mnk substrate in vivo. We also demonstrate that PSF, together with its partner, p54nrb, binds RNAs that contain AU-rich elements (AREs), such as those for proinflammatory cytokines (e.g. tumor necrosis factor α (TNFα)). Indeed, PSF associates specifically with the TNFα mRNA in living cells. PSF is phosphorylated at two sites by the Mnks. Our data show that Mnk-mediated phosphorylation increases the binding of PSF to the TNFα mRNA in living cells. These findings identify a novel Mnk substrate. They also suggest that the Mnk-catalyzed phosphorylation of PSF may regulate the fate of specific mRNAs by modulating their binding to PSF·p54nrb. |
Keywords: | Cell Line Jurkat Cells Humans Tumor Necrosis Factor-alpha RNA-Binding Proteins DNA-Binding Proteins Nuclear Matrix-Associated Proteins RNA, Messenger 3' Untranslated Regions Immunoblotting Chromatography, Affinity Electrophoresis, Polyacrylamide Gel Transfection Reverse Transcriptase Polymerase Chain Reaction Immunoprecipitation Protein Binding Substrate Specificity Phosphorylation Octamer Transcription Factors PTB-Associated Splicing Factor Protein Serine-Threonine Kinases |
Rights: | © 2008 by The American Society for Biochemistry and Molecular Biology, Inc. |
DOI: | 10.1074/jbc.M705286200 |
Published version: | http://dx.doi.org/10.1074/jbc.m705286200 |
Appears in Collections: | Aurora harvest 4 Molecular and Biomedical Science publications |
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