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https://hdl.handle.net/2440/8399
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dc.contributor.author | Jasper, M. | - |
dc.contributor.author | Brannstrom, M. | - |
dc.contributor.author | Olofsson, J. | - |
dc.contributor.author | Petrucco, O. | - |
dc.contributor.author | Mason, H. | - |
dc.contributor.author | Robertson, S. | - |
dc.contributor.author | Norman, R. | - |
dc.date.issued | 1996 | - |
dc.identifier.citation | Molecular Human Reproduction, 1996; 2(8):555-562 | - |
dc.identifier.issn | 1360-9947 | - |
dc.identifier.issn | 1460-2407 | - |
dc.identifier.uri | http://hdl.handle.net/2440/8399 | - |
dc.description | © European Society of Human Reproduction and Embryology 1996 | - |
dc.description.abstract | In recent years it has become evident that a leukocyte-cytokine network contributes to the paracrine regulation of ovarian function. The objectives of this study were to examine the presence of a potent lympho-haemopoietic cytokine, granulocyte-macrophage colony-stimulating factor (GM-CSF), in tissues and fluids from human ovaries. In a prospective study, follicular fluid and plasma were collected from naturally cycling women and women undergoing hyperstimulation for in-vitro fertilization (IVF). Granulosa-lutein cells were collected at the time of oocyte recovery for IVF and corpora lutea were collected at the time of hysterectomy for non-ovarian reasons. Culture supernatants from ovarian cell and tissue cultures were harvested on completion of a 48 h incubation. Immunoactive GM-CSF was measured by enzyme-linked immunosorbent assay, and was found to be present at statistically significantly higher levels in follicular fluid (8.9 +/- 0.7 pg/ml) and plasma (11.3 +/- 0.8 pg/ml) of women undergoing hyperstimulation compared to follicular fluid (5.3 +/- 0.3 pg/ml) and plasma (7.1 +/- 0.5 pg/ml) from naturally cycling women. Immunoactive GM-CSF was also detected in culture supernatants of granulosa-lutein cells (47.6 pg/10(5) cells), early luteal phase corpora lutea (0.52 pg/microgram DNA) and mid-luteal phase corpora lutea (0.98 pg/microgram DNA). Furthermore, transcripts for GM-CSF, and both the alpha and beta subunits of the GM-CSF receptor, were detected by reverse transcription polymerase chain reaction (RT-PCR) in granulosa-lutein cell culture preparations and corpora lutea collected during the early, mid- and late luteal phase of the menstrual cycle. These results show that GM-CSF is expressed and secreted by cells within the human ovary, and, together with the finding of expression of mRNA for GM-CSF receptor, suggest a role for GM-CSF in the local regulation of ovarian events. | - |
dc.description.statementofresponsibility | Melinda J. Jasper, Mats Brännström, Jan I. OIofsson, Ossie M. Petrucco, Helen Mason, Sarah A. Robertson and Robert J. Norman | - |
dc.language.iso | en | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.source.uri | http://dx.doi.org/10.1093/molehr/2.8.555 | - |
dc.subject | Ovary | - |
dc.subject | Corpus Luteum | - |
dc.subject | Luteal Cells | - |
dc.subject | Follicular Fluid | - |
dc.subject | Granulosa Cells | - |
dc.subject | Cells, Cultured | - |
dc.subject | Humans | - |
dc.subject | Granulocyte-Macrophage Colony-Stimulating Factor | - |
dc.subject | Receptors, Granulocyte-Macrophage Colony-Stimulating Factor | - |
dc.subject | RNA, Messenger | - |
dc.subject | DNA Primers | - |
dc.subject | Polymerase Chain Reaction | - |
dc.subject | Gene Expression | - |
dc.subject | Base Sequence | - |
dc.subject | Protein Conformation | - |
dc.subject | Adult | - |
dc.subject | Female | - |
dc.subject | In Vitro Techniques | - |
dc.title | Granulocyte-macrophage colony-stimulating factor: presence in human follicular fluid, protein secretion and mRNA expression by ovarian cells | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1093/molehr/2.8.555 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Robertson, S. [0000-0002-9967-0084] | - |
dc.identifier.orcid | Norman, R. [0000-0002-3118-3896] | - |
Appears in Collections: | Aurora harvest 4 Obstetrics and Gynaecology publications |
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