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https://hdl.handle.net/2440/8411
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Type: | Journal article |
Title: | Role of germinal vesicle on protein synthesis in rat oocyte during in vitro maturation |
Author: | Meng, L. Rutledge, J. Zhu, Y. Kidder, G. Khamsi, F. Armstrong, D. |
Citation: | Molecular Reproduction and Development, 1996; 43(2):228-235 |
Issue Date: | 1996 |
ISSN: | 1040-452X 1098-2795 |
Statement of Responsibility: | Li Meng, Jean Rutledge, Ying Zhu, Gerald M. Kidder, Firouz Khamsi, David T. Armstrong |
Abstract: | To investigate the role of the germinal vesicle (GV) on in vitro maturation (IVM) of rat oocytes, we examined protein synthesis during IVM by comparing polypeptide patterns in control and enucleated oocytes using one and two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Separation of polypeptides extracted from the cytoplasm of GV by one-dimensional SDS-PAGE revealed that a 55 kDa polypeptide was present only in the GVs of rat oocytes. At 0, 12, 24, 36, and 44 hr after PMSG injection, prior to the initiation of maturation, enucleated oocytes synthesized the same major polypeptides as cumulus intact (CI) oocytes. During meiotic maturation, no major changes were detected in protein synthesis from prophase (GV stage) to prometaphase I (0-6 hr IVM). However, after entry into prometaphase I (7 hr IVM), striking changes were seen; a 24 kDa polypeptide disappeared and expression of a 34 kDa polypeptide became stronger. This pattern lasted until metaphase II. We detected no major differences in the pattern of protein synthesis between CI and enucleated oocytes using two-dimensional PAGE. These results indicate that protein synthesis in the maturing rat oocyte is controlled by cytoplasmic regulators rather than intrinsic nuclear components. |
Keywords: | Oocytes Cytoplasmic Granules Animals Rats Rats, Sprague-Dawley Cell Differentiation Protein Biosynthesis Female In Vitro Techniques |
Rights: | Copyright © 1996 Wiley-Liss, Inc. |
DOI: | 10.1002/(SICI)1098-2795(199602)43:2<228::AID-MRD12>3.0.CO;2 |
Appears in Collections: | Aurora harvest 4 Obstetrics and Gynaecology publications |
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