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https://hdl.handle.net/2440/85258
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dc.contributor.author | Wang, L. | - |
dc.contributor.author | Robertson, S.A. | - |
dc.contributor.author | Seamark, R.F. | - |
dc.contributor.author | Norman, R.J. | - |
dc.date.issued | 1991 | - |
dc.identifier.citation | Journal of Clinical Endocrinology and Metabolism, 1991; 72(4):824-831 | - |
dc.identifier.issn | 0021-972X | - |
dc.identifier.issn | 1945-7197 | - |
dc.identifier.uri | http://hdl.handle.net/2440/85258 | - |
dc.description.abstract | The effects of human interleukin-1 (IL-1) and IL-2 on human granulosa-luteal cell progesterone production were examined with or without hCG stimulation in vitro. Human granulosa-luteal cells were recovered from follicular fluid obtained from women undergoing in vitro fertilization procedures and cultured for up to 7 days before supernatant progesterone level measurement. Lymphokine-rich conditioned medium was prepared from mitogen-stimulated human peripheral blood leukocytes (HPL-CM). The influence of HPL-CM on both granulosa-luteal cell progesterone production and cell growth was inhibitory. In contrast, supernatants of the IL-2-producing cell line MLA-144 (MLA-CM) stimulated both basal progesterone secretion and cell proliferation. Human recombinant IL-2 (from 0.1-100 IU) alone did not change progesterone levels, compared to control values, after 24 h of cell culture. However, 1, 10, and 100 IU IL-2 significantly inhibited progesterone secretion from cells stimulated by 5 IU hCG (P less than 0.01). The enhanced progesterone levels stimulated by forskolin were also significantly inhibited by 10 IU IL-2 (P = 0.01). This effect was not mediated through decreased cAMP, since the forskolin-enhanced cAMP level was not influenced by IL-2, IL-1, with or without hCG, did not show any effect on progesterone production during either 24 or 48 h of cell culture. It is concluded that 1) human recombinant IL-2 significantly inhibits progesterone production stimulated by hCG in human granulosa-luteal cells; 2) IL-2 also had a marked inhibitory effect on forskolin-induced progesterone release, but did not influence the increased cAMP level stimulated by forskolin; 3) the inhibitory influence of IL-2 on progesterone synthesis may be down-stream in the signal transduction pathway from cAMP activation; and 4) HPL-CM and MLA-CM produced inhibitory and stimulatory effects, respectively, on both basal and hCG-stimulated progesterone levels as well as on granulosa-luteal cell proliferation. These activities cannot be completely attributed to IL-2, and other mediators of leukocyte origin may, therefore, exist. | - |
dc.description.statementofresponsibility | Lingjia Wang, Sarah Robertson, Robert F. Seamark, and Robert J. Norman | - |
dc.language.iso | en | - |
dc.publisher | Endocrine Society | - |
dc.rights | Copyright © 1991 by The Endocrine Society | - |
dc.source.uri | http://dx.doi.org/10.1210/jcem-72-4-824 | - |
dc.subject | Corpus Luteum | - |
dc.subject | Granulosa Cells | - |
dc.subject | Cells, Cultured | - |
dc.subject | Humans | - |
dc.subject | Progesterone | - |
dc.subject | Chorionic Gonadotropin | - |
dc.subject | Interleukin-1 | - |
dc.subject | Interleukin-2 | - |
dc.subject | Lymphokines | - |
dc.subject | Culture Media | - |
dc.subject | Cell Division | - |
dc.subject | Female | - |
dc.subject | Colforsin | - |
dc.title | Lymphokines, including interleukin-2, alter gonadotropin-stimulated progesterone production and proliferation of human granulosa-luteal cells in vitro | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1210/jcem-72-4-824 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Robertson, S.A. [0000-0002-9967-0084] | - |
dc.identifier.orcid | Norman, R.J. [0000-0002-3118-3896] | - |
Appears in Collections: | Aurora harvest 7 Obstetrics and Gynaecology publications |
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