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https://hdl.handle.net/2440/85554
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Type: | Journal article |
Title: | Altered immunity and dendritic cell activity in the periphery of mice after long-term engraftment with bone marrow from ultraviolet-irradiated mice |
Author: | Ng, R. Scott, N. Strickland, D. Gorman, S. Grimbaldeston, M. Norval, M. Waithman, J. Hart, P. |
Citation: | Journal of Immunology, 2013; 190(11):5471-5484 |
Publisher: | American Association of Immunologists |
Issue Date: | 2013 |
ISSN: | 0022-1767 1550-6606 |
Statement of Responsibility: | Royce L. X. Ng, Naomi M. Scott, Deborah H. Strickland, Shelley Gorman, Michele A. Grimbaldeston, Mary Norval, Jason Waithman and Prue H. Hart |
Abstract: | Alterations to dendritic cell (DC) progenitors in the bone marrow (BM) may contribute to long-lasting systemic immunosuppression (>28 d) following exposure of the skin of mice to erythemal UV radiation (UVR). DCs differentiated in vitro from the BM of mice 3 d after UVR (8 kJ/m2) have a reduced capacity to initiate immunity (both skin and airways) when adoptively transferred into naive mice. Studies in IL-10−/− mice suggested that UV-induced IL-10 was not significantly involved. To investigate the immune capabilities of peripheral tissue DCs generated in vivo from the BM of UV-irradiated mice, chimeric mice were established. Sixteen weeks after reconstitution, contact hypersensitivity responses were significantly reduced in mice reconstituted with BM from UV-irradiated mice (UV-chimeric). When the dorsal skin of UV-chimeric mice was challenged with innate inflammatory agents, the hypertrophy induced in the draining lymph nodes was minimal and significantly less than that measured in control-chimeric mice challenged with the same inflammatory agent. When DCs were differentiated from the BM of UV-chimeric mice using FLT3 ligand or GM-CSF + IL-4, the cells maintained a reduced priming ability. The diminished responses in UV-chimeric mice were not due to different numerical or proportional reconstitution of BM or the hematopoietic cells in blood, lymph nodes, and skin. Erythemal UVR may imprint a long-lasting epigenetic effect on DC progenitors in the BM and alter the function of their terminally differentiated progeny. |
Keywords: | Lymph Nodes Dendritic Cells T-Lymphocytes Bone Marrow Cells Animals Mice Dermatitis, Contact Hypertrophy Granulocyte-Macrophage Colony-Stimulating Factor Membrane Proteins Interleukin-4 Adoptive Transfer Bone Marrow Transplantation Ultraviolet Rays Cell Differentiation Cell Movement Graft Survival Chimerism Female Immunity, Innate |
Rights: | © 2013 by The American Association of Immunologists, Inc. |
DOI: | 10.4049/jimmunol.1202786 |
Grant ID: | 1011203 539800 |
Published version: | http://dx.doi.org/10.4049/jimmunol.1202786 |
Appears in Collections: | Aurora harvest 7 Molecular and Biomedical Science publications |
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