Voltage dependence of Hodgkin-Huxley rate functions for a multistage K⁺ channel voltage sensor within a membrane

Abstract

The activation of a K+ channel sensor in two sequential stages during a voltage clamp may be described as the translocation of a Brownian particle in an energy landscape with two large barriers between states. A solution of the Smoluchowski equation for a square-well approximation to the potential function of the S4 voltage sensor satisfies a master equation, and has two frequencies that may be determined from the forward and backward rate functions. When the higher frequency terms have small amplitude, the solution reduces to the relaxation of a rate equation, where the derived two-state rate functions are dependent on the relative magnitude of the forward rates ($\alpha$ and $\gamma$) and the backward rates ($\beta$ and $\delta$) for each stage. In particular, the voltage dependence of the Hodgkin-Huxley rate functions for a K+ channel may be derived by assuming that the rate functions of the first stage are large relative to those of the second stage - $\alpha \gg \gamma $ and $\beta \gg \delta$. For a {\em Shaker} IR K+ channel, the first forward and backward transitions are rate limiting ($\alpha < \gamma $ and $\delta \ll \beta$), and for an activation process with either two or three stages, the derived two-state rate functions also have a voltage dependence that is of a similar form to that determined for the squid axon. The potential variation generated by the interaction between a two-stage K+ ion channel and a noninactivating $Na^+$ ion channel is determined by the master equation for K+ channel activation and the ionic current equation when the Na+ channel activation time is small, and if $\beta \ll \delta $ and $\alpha \ll \gamma $, the system may exhibit a small amplitude oscillation between spikes, or mixed-mode oscillation, in which the slow closed state modulates the K+ ion channel conductance in the membrane.