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https://hdl.handle.net/2440/88257
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Type: | Journal article |
Title: | Metal-containing polystyrene beads as standards for mass cytometry |
Author: | Abdelrahman, A. Ornatsky, O. Bandura, D. Baranov, V. Kinach, R. Dai, S. Thickett, S. Tanner, S. Winnik, M. |
Citation: | Journal of Analytical Atomic Spectrometry, 2010; 25(3):260-268 |
Publisher: | Royal Society of Chemistry |
Issue Date: | 2010 |
ISSN: | 0267-9477 1364-5544 |
Statement of Responsibility: | Ahmed I. Abdelrahman, Olga Ornatsky, Dmitry Bandura, Vladimir Baranov, Robert Kinach, Sheng Dai, Stuart C. Thickett, Scott Tanner and Mitchell A. Winnik |
Abstract: | We examine the suitability of metal-containing polystyrene beads for the calibration of a mass cytometer instrument, a single particle analyser based on an inductively coupled plasma ion source and a time of flight mass spectrometer. These metal-containing beads are also verified for their use as internal standards for this instrument. These beads were synthesized by multiple-stage dispersion polymerization with acrylic acid as a comonomer. Acrylic acid acts as a ligand to anchor the metal ions within the interior of the beads. Mass cytometry enabled the bead-by-bead measurement of the metal-content and determination of the metal-content distribution. Beads synthesized by dispersion polymerization that involved three stages were shown to have narrower bead-to-bead variation in their lanthanide content than beads synthesized by 2-stage dispersion polymerization. The beads exhibited insignificant release of their lanthanide content to aqueous solutions of different pHs over a period of six months. When mixed with KG1a or U937 cell lines, metal-containing polymer beads were shown not to affect the mass cytometry response to the metal content of element-tagged antibodies specifically attached to these cells. |
Rights: | ©The Royal Society of Chemistry 2010 |
DOI: | 10.1039/b921770c |
Published version: | http://dx.doi.org/10.1039/b921770c |
Appears in Collections: | Aurora harvest 2 Chemistry publications |
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