Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/89171
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Type: | Journal article |
Title: | Genotyping single nucleotide polymorphisms using different molecular beacon multiplexed within a suspended core optical fiber |
Author: | Linh, V. Giannetti, S. Warren-Smith, S. Cooper, A. Selleri, S. Cucinotta, A. Monro, T. |
Citation: | Sensors, 2014; 14(8):14488-14499 |
Publisher: | MDPI AG |
Issue Date: | 2014 |
ISSN: | 1424-8220 1424-8220 |
Statement of Responsibility: | Linh Viet Nguyen, Sara Giannetti, Stephen Warren-Smith, Alan Cooper, Stefano Selleri, Annamaria Cucinotta, and Tanya Monro |
Abstract: | We report a novel approach to genotyping single nucleotide polymorphisms (SNPs) using molecular beacons in conjunction with a suspended core optical fiber (SCF). Target DNA sequences corresponding to the wild- or mutant-type have been accurately recognized by immobilizing two different molecular beacons on the core of a SCF. The two molecular beacons differ by one base in the loop-probe and utilize different fluorescent indicators. Single-color fluorescence enhancement was obtained when the immobilized SCFs were filled with a solution containing either wild-type or mutant-type sequence (homozygous sample), while filling the immobilized SCF with solution containing both wild- and mutant-type sequences resulted in dual-color fluorescence enhancement, indicating a heterozygous sample. The genotyping was realized amplification-free and with ultra low-volume for the required DNA solution (nano-liter). This is, to our knowledge, the first genotyping device based on the combination of optical fiber and molecular beacons. |
Keywords: | DNA Genotype Polymorphism, Single Nucleotide Fluorescence Optical Fibers Genotyping Techniques |
Rights: | © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
DOI: | 10.3390/s140814488 |
Published version: | http://dx.doi.org/10.3390/s140814488 |
Appears in Collections: | Aurora harvest 2 Ecology, Evolution and Landscape Science publications |
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hdl_89171.pdf | Published version | 991.06 kB | Adobe PDF | View/Open |
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