Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/89171
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dc.contributor.author | Linh, V. | - |
dc.contributor.author | Giannetti, S. | - |
dc.contributor.author | Warren-Smith, S. | - |
dc.contributor.author | Cooper, A. | - |
dc.contributor.author | Selleri, S. | - |
dc.contributor.author | Cucinotta, A. | - |
dc.contributor.author | Monro, T. | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | Sensors, 2014; 14(8):14488-14499 | - |
dc.identifier.issn | 1424-8220 | - |
dc.identifier.issn | 1424-8220 | - |
dc.identifier.uri | http://hdl.handle.net/2440/89171 | - |
dc.description.abstract | We report a novel approach to genotyping single nucleotide polymorphisms (SNPs) using molecular beacons in conjunction with a suspended core optical fiber (SCF). Target DNA sequences corresponding to the wild- or mutant-type have been accurately recognized by immobilizing two different molecular beacons on the core of a SCF. The two molecular beacons differ by one base in the loop-probe and utilize different fluorescent indicators. Single-color fluorescence enhancement was obtained when the immobilized SCFs were filled with a solution containing either wild-type or mutant-type sequence (homozygous sample), while filling the immobilized SCF with solution containing both wild- and mutant-type sequences resulted in dual-color fluorescence enhancement, indicating a heterozygous sample. The genotyping was realized amplification-free and with ultra low-volume for the required DNA solution (nano-liter). This is, to our knowledge, the first genotyping device based on the combination of optical fiber and molecular beacons. | - |
dc.description.statementofresponsibility | Linh Viet Nguyen, Sara Giannetti, Stephen Warren-Smith, Alan Cooper, Stefano Selleri, Annamaria Cucinotta, and Tanya Monro | - |
dc.language.iso | en | - |
dc.publisher | MDPI AG | - |
dc.rights | © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). | - |
dc.source.uri | http://dx.doi.org/10.3390/s140814488 | - |
dc.subject | DNA | - |
dc.subject | Genotype | - |
dc.subject | Polymorphism, Single Nucleotide | - |
dc.subject | Fluorescence | - |
dc.subject | Optical Fibers | - |
dc.subject | Genotyping Techniques | - |
dc.title | Genotyping single nucleotide polymorphisms using different molecular beacon multiplexed within a suspended core optical fiber | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.3390/s140814488 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Linh, V. [0000-0002-2543-2374] | - |
dc.identifier.orcid | Warren-Smith, S. [0000-0002-2612-6344] | - |
dc.identifier.orcid | Cooper, A. [0000-0002-7738-7851] | - |
Appears in Collections: | Aurora harvest 2 Ecology, Evolution and Landscape Science publications |
Files in This Item:
File | Description | Size | Format | |
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hdl_89171.pdf | Published version | 991.06 kB | Adobe PDF | View/Open |
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