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https://hdl.handle.net/2440/89264
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Type: | Journal article |
Title: | Characterization of bone marrow derived mesenchymal stem cells in suspension |
Author: | Akiyama, K. You, Y. Yamaza, T. Chen, C. Tang, L. Jin, Y. Chen, X. Gronthos, S. Shi, S. |
Citation: | Stem Cell Research and Therapy, 2012; 3(5):40-1-40-13 |
Publisher: | BioMed Central |
Issue Date: | 2012 |
ISSN: | 1757-6512 1757-6512 |
Statement of Responsibility: | Kentaro Akiyama, Yong-Ouk You, Takayoshi Yamaza, Chider Chen, Liang Tang, Yan Jin, Xiao-Dong Chen, Stan Gronthos, and Songtao Shi |
Abstract: | INTRODUCTION: Bone marrow mesenchymal stem cells (BMMSCs) are a heterogeneous population of postnatal precursor cells with the capacity of adhering to culture dishes generating colony-forming unit-fibroblasts (CFU-F). Here we identify a new subset of BMMSCs that fail to adhere to plastic culture dishes and remain in culture suspension (S-BMMSCs). METHODS: To catch S-BMMSCs, we used BMMSCs-produced extracellular cell matrix (ECM)-coated dishes. Isolated S-BMMSCs were analyzed by in vitro stem cell analysis approaches, including flow cytometry, inductive multiple differentiation, western blot and in vivo implantation to assess the bone regeneration ability of S-BMMSCs. Furthermore, we performed systemic S-BMMSCs transplantation to treat systemic lupus erythematosus (SLE)-like MRL/lpr mice. RESULTS: S-BMMSCs are capable of adhering to ECM-coated dishes and showing mesenchymal stem cell characteristics with distinction from hematopoietic cells as evidenced by co-expression of CD73 or Oct-4 with CD34, forming a single colony cluster on ECM, and failure to differentiate into hematopoietic cell lineage. Moreover, we found that culture-expanded S-BMMSCs exhibited significantly increased immunomodulatory capacities in vitro and an efficacious treatment for SLE-like MRL/lpr mice by rebalancing regulatory T cells (Tregs) and T helper 17 cells (Th17) through high NO production. CONCLUSIONS: These data suggest that it is feasible to improve immunotherapy by identifying a new subset BMMSCs. |
Keywords: | Bone Marrow Cells Stem Cells Mesenchymal Stem Cells Animals Mice, Inbred C3H Mice, Inbred C57BL Humans Mice Mice, Nude Cell Proliferation Female |
Rights: | © 2012 Akiyama et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
DOI: | 10.1186/scrt131 |
Published version: | http://dx.doi.org/10.1186/scrt131 |
Appears in Collections: | Aurora harvest 2 Medical Sciences publications |
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hdl_89264.pdf | Published version | 3.26 MB | Adobe PDF | View/Open |
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