Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/89556
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Full metadata record
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dc.contributor.author | Purdey, M. | - |
dc.contributor.author | Connaughton, H. | - |
dc.contributor.author | Whiting, S. | - |
dc.contributor.author | Schartner, E. | - |
dc.contributor.author | Monro, T. | - |
dc.contributor.author | Thompson, J. | - |
dc.contributor.author | John Aitken, R. | - |
dc.contributor.author | Abell, A. | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | Free Radical Biology and Medicine, 2015; 81:69-76 | - |
dc.identifier.issn | 0891-5849 | - |
dc.identifier.issn | 1873-4596 | - |
dc.identifier.uri | http://hdl.handle.net/2440/89556 | - |
dc.description.abstract | Human spermatozoa are compromised by production of reactive oxygen species (ROS) and detection of ROS in spermatozoa is important for the diagnosis of male infertility. Probes 2',7'-dichlorohydrofluorescein diacetate (DCFH), dihydroethidium (DHE) and MitoSOX red (MSR) are commonly used for detecting ROS by flow cytometry, however these probes lack sensitivity to hydrogen peroxide (H2O2), which is particularly damaging to mammalian sperm cells. This study reports the synthesis and use of three aryl boronate probes, peroxyfluor-1 (PF1), carboxy peroxyfluor-1 (CPF1) and a novel probe 2(2-ethoxyethoxy)ethoxy peroxyfluor-1 (EEPF1) in human spermatozoa. PF1 and EEPF1 were found to be effective in detecting H2O2 and peroxynitrite (ONOO(-)) produced by spermatozoa when stimulated with menadione or 4-hydroxynonenal. EEPF1 was more effective at detection of ROS in spermatozoa than DCFH, DHE and MSR; furthermore it distinguished poorly motile sperm as shown by greater ROS production. EEPF1 should therefore have a significant role in diagnosis of oxidative stress in male infertility, cryopreservation, age, lifestyle and exposure to environmental toxicants. | - |
dc.description.statementofresponsibility | Malcolm S. Purdey, Haley S. Connaughton, Sara Whiting, Erik P. Schartner, Tanya M. Monro, Jeremy G. Thompson, R. John Aitken, Andrew D. Abell | - |
dc.language.iso | en | - |
dc.publisher | Elsevier | - |
dc.rights | © 2015 Elsevier Inc. | - |
dc.source.uri | http://dx.doi.org/10.1016/j.freeradbiomed.2015.01.015 | - |
dc.subject | Aryl boronate | - |
dc.subject | Free radicals | - |
dc.subject | Human spermatozoa | - |
dc.subject | Hydrogen peroxide | - |
dc.subject | Organic synthesis | - |
dc.subject | Reactive oxygen species | - |
dc.title | Boronate probes for the detection of hydrogen peroxide release from human spermatozoa | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1016/j.freeradbiomed.2015.01.015 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/LP110200736 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/DP110103951 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/FL130100044 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Purdey, M. [0000-0002-5063-8972] | - |
dc.identifier.orcid | Schartner, E. [0000-0003-1669-4302] | - |
dc.identifier.orcid | Thompson, J. [0000-0003-4941-7731] | - |
dc.identifier.orcid | Abell, A. [0000-0002-0604-2629] | - |
Appears in Collections: | Aurora harvest 2 Paediatrics publications |
Files in This Item:
File | Description | Size | Format | |
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hdl_89556.pdf | Accepted version | 325.15 kB | Adobe PDF | View/Open |
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