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https://hdl.handle.net/2440/89602
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Type: | Journal article |
Title: | Colpoda secrete viable Listeria monocytogenes within faecal pellets |
Author: | Raghu Nadhanan, R. Thomas, C. |
Citation: | Environmental Microbiology, 2014; 16(2):396-404 |
Publisher: | Blackwell Science |
Issue Date: | 2014 |
ISSN: | 1462-2920 1462-2912 |
Statement of Responsibility: | Rethish Raghu Nadhanan and Connor J. Thomas |
Abstract: | Transmission electron microscopy was used to demonstrate that co-cultures of the ciliate Colpoda RR (an environmental isolate) and Colpoda MLS-5 (a food processing environment isolate) with the pathogenic Listeria monocytogenes DRDC8 resulted in secretion of faecal pellets containing intact DRDC8 cells. A green fluorescent protein expressing variant of DRDC8 was used in co-cultures to confirm that the pellet-associated bacterial cells were L.monocytogenes. Viability was confirmed by plate counts, and assay of microbial respiratory activity-proved DRDC8 cells present within faecal pellets was metabolically active. Following treatment of faecal pellets secreted by Colpoda RR and MLS-5 with gentamycin and sodium hypochlorite (NaOCl), no loss of viability of the pellet-located DRDC8 cells was observed, indicating that faecal pellet encapsulated DRDC8 cells are resistant to biocidal agents. This work suggests that Colpoda-derived faecal pellets may provide a mechanism for transmission of L.monocytogenes and other pathogenic bacteria. Furthermore, bacteria encapsulated by faecal pellets may be resistant to disinfectants and cleaning agents used in food manufacturing and preparation facilities. |
Keywords: | Ciliophora Listeria monocytogenes Sodium Hypochlorite Gentamicins Disinfectants Microscopy, Electron, Transmission Coculture Techniques Food Handling Microbial Viability |
Rights: | © 2013 Society for Applied Microbiology and John Wiley & Sons Ltd |
DOI: | 10.1111/1462-2920.12230 |
Published version: | http://dx.doi.org/10.1111/1462-2920.12230 |
Appears in Collections: | Aurora harvest 2 Molecular and Biomedical Science publications |
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