Adelaide Research & Scholarship
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https://hdl.handle.net/2440/90508
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| Type: | Journal article |
| Title: | Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus |
| Author: | Christo, S. Diener, K. Nordon, R. Brown, M. Griesser, H. Vasilev, K. Christo, F. Hayball, J. |
| Citation: | Scientific Reports, 2015; 5(1):7760-1-7760-8 |
| Publisher: | Nature Publishing Group |
| Issue Date: | 2015 |
| ISSN: | 2045-2322 2045-2322 |
| Statement of Responsibility: | Susan N. Christo, Kerrilyn R. Diener, Robert E. Nordon, Michael P. Brown, Hans J. Griesser, Krasimir Vasilev, Farid C. Christo, John D. Hayball |
| Abstract: | The capture and activation of individual T cells on functionalised surfaces enables real-time analyses of the magnitude and rhythm of intracellular calcium release. Application of Haarlet transformations generate a calcium flux 'threshold', with the frequency of the 'threshold crossings' correlating with the strength of the original T cell stimulus. These findings represent a new method to evaluate graduations in T cell activation in real time, and at a single-cell level. |
| Keywords: | T-Lymphocytes Animals Mice, Inbred C57BL Receptors, Antigen, T-Cell Ligands Lymphocyte Activation Calcium Signaling Fluorescence CD3 Complex CD28 Antigens |
| Rights: | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| DOI: | 10.1038/srep07760 |
| Published version: | http://dx.doi.org/10.1038/srep07760 |
| Appears in Collections: | Aurora harvest 2 Paediatrics publications |
Files in This Item:
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| hdl_90508.pdf | Published version | 1.08 MB | Adobe PDF | View/Open |
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