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https://hdl.handle.net/2440/95892
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Type: | Journal article |
Title: | Milk as a diagnostic sample for a commercially available ELISA to identify bovine viral diarrhoea (BVD) antibodies in dairy herds |
Author: | Lanyon, S. McCoy, R. Bergman, E. Reichel, M. |
Citation: | Australian Veterinary Journal, 2014; 92(7):269-273 |
Publisher: | Wiley |
Issue Date: | 2014 |
ISSN: | 0005-0423 1751-0813 |
Statement of Responsibility: | SR Lanyon, R McCoy, E Bergman and MP Reichel |
Abstract: | OBJECTIVE: The aims of this study were to evaluate a commercially available ELISA for the detection of bovine viral diarrhoea virus (BVDV)-specific antibodies in individual milk compared with individual serum samples, and in bulk milk samples compared with within-herd antibody prevalence and bulk milk quantitative reverse transcription polymerase chain reaction (qRT-PCR) results. METHODS: Paired individual serum and individual milk samples were collected from 125 lactating cows and tested by ELISA; 96 bulk milk samples were also tested. Within-herd antibody prevalence was calculated based on milk ELISA results for 25 individual cows in each herd. Additionally, 167 bulk milk samples were tested for BVDV-specific antibodies by ELISA and for the presence of BVDV by qRT-PCR to establish the correlation between antibody result and virus presence. RESULTS: Good agreement was observed between individual milk and serum results (Kappa = 0.865). The ELISA was observed to detect BVDV-specific antibodies in individual milk samples with a relative sensitivity of 96.6% and specificity of 89.2%. The bulk milk samples revealed a strong (r(2) = 0.95) relationship between the ELISA result and the within-herd antibody prevalence. The proportion of herds that tested positive by bulk milk qRT-PCR increased as the bulk milk antibody S/P ratio increased. CONCLUSION: Commercially available ELISA testing of individual and bulk milk samples is an appropriate alternative to serum testing with good test performance in these samples. Determining a threshold for the detection of herds containing active BVD infection by testing bulk milk is a novel use for an antibody ELISA kit and provides more practical, relevant test results. |
Keywords: | bovine viral diarrhoea; ELISA; milk; PCR |
Rights: | © 2014 Australian Veterinary Association |
DOI: | 10.1111/avj.12188 |
Published version: | http://dx.doi.org/10.1111/avj.12188 |
Appears in Collections: | Animal and Veterinary Sciences publications Aurora harvest 3 |
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