The organic P composition of vertisols as determined by ³¹P NMR spectroscopy

Abstract

Solution ³¹P nuclear magnetic resonance (NMR) spectroscopy on sodium hydroxide–ethylenediaminetetraacetic acid (NaOH–EDTA) extracts can provide detailed characterization of soil organic P, but has not been previously applied widely to Vertisols. Vertisol soils were collected at two depths (0–10 cm and 10–30 cm) for chemical and spectroscopic analysis. Sodium hydroxide–EDTA extracted a relatively consistent proportion (17 to 37%; average 25%) of the total organic P content determined by the ignition–H₂SO₄ extraction technique. Orthophosphate monoesters were the dominant form of organic P detected by solution ³¹P NMR spectroscopy of the NaOH–EDTA extracts, the majority of which appeared to be present in large ‘humic’ molecules, based on the predominance of a broad peak in the NMR spectra; some smaller signals due to glycerophosphate, inositol phosphates, and RNA-derived mononucleotides were also evident. The composition of organic P in the topsoil layer was very similar to that in the subsoil layer. Strong correlations were found between soil organic carbon (C) and nitrogen (N) and extractable organic P, which suggests that processes regulating the cycling of organic C and N are closely related to that for organic P in these Vertisols.