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https://hdl.handle.net/2440/103228
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Type: | Journal article |
Title: | Enhancing enzyme stability and metabolic functional ability of β-galactosidase through functionalized polymer nanofiber immobilization |
Other Titles: | Enhancing enzyme stability and metabolic functional ability of beta-galactosidase through functionalized polymer nanofiber immobilization |
Author: | Misson, M. Jin, B. Chen, B. Zhang, H. |
Citation: | Bioprocess and Biosystems Engineering: bioreactors, upstream and downstream processes, measurement and control, 2015; 38(10):1915-1923 |
Publisher: | Springer |
Issue Date: | 2015 |
ISSN: | 1615-7591 1615-7605 |
Statement of Responsibility: | Mailin Misson, Bo Jin, Binghui Chen, Hu Zhang |
Abstract: | A functionalized polystyrene nanofiber (PSNF) immobilized β-galactosidase assembly (PSNF-Gal) was synthesized as a nanobiocatalyst aiming to enhance the biocatalyst stability and functional ability. The PSNF fabricated by electrospinning was functionalized through a chemical oxidation method for enzyme binding. The bioengineering performance of the enzyme carriers was further evaluated for bioconversion of lactose to galacto-oligosaccharides (GOS). The modified PSNF-Gal demonstrated distinguished performances to preserve the same activity as the free β-galactosidase at the optimum pH of 7.0, and to enhance the enzyme stability of PSNF-Gal in an alkaline condition up to pH 10. The PSNF assembly demonstrated improved thermal stability from 37 to 60°C. The nanobiocatalyst was able to retain 30% of its initial activity after ninth operation cycles comparing to four cycles with the unmodified counterpart. In contrast with free β-galactosidase, the modified PSNF-Gal enhanced the GOS yield from 14 to 28%. These findings show the chemically modified PSNF-based nanobiocatalyst may be pertinent for various enzyme-catalysed bioprocessing applications. |
Keywords: | Nanobiocatalysts; galacto-oligosaccharides; β-galactosidase; polymer nanofibers; bioconversion |
Rights: | © Springer-Verlag Berlin Heidelberg 2015 |
DOI: | 10.1007/s00449-015-1432-5 |
Published version: | http://dx.doi.org/10.1007/s00449-015-1432-5 |
Appears in Collections: | Aurora harvest 3 Chemical Engineering publications |
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