Synthesis of complex oligosaccharides by using a mutated (1,3)-beta-D-glucan endohydrolase from barley

Abstract

Complex oligosaccharides with newly formed (1,3)-β-glycosidic linkages were obtained in good to excellent yields when substituted or unsubstituted α-laminaribiosyl fluorides, acting as donors, were condensed onto mono- and disaccharide β-D-hexopyranoside acceptors by using a (1,3)-β-D-glycosynthase. These linear and branched (1,3)-β-linked oligosaccharides could prove to be important in a range of medical, pharmaceutical, and agricultural applications. Furthermore, the observation that the (1,3)-β-D-glucan glycosynthase accommodates (1,3)-, (1,4), - and (1,6)-β-oligosaccharides in its acceptor subsites suggests novel, yet unexpected physiological roles for the wild type (1,3)-β-D-glucan endohydrolase from higher plants.