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https://hdl.handle.net/2440/114947
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dc.contributor.author | Kok, T.W. | - |
dc.contributor.author | Varkanis, G. | - |
dc.contributor.author | Marmion, B.P. | - |
dc.contributor.author | Martin, J. | - |
dc.contributor.author | Esterman, A. | - |
dc.date.issued | 1988 | - |
dc.identifier.citation | Epidemiology and Infection, 1988; 101(3):669-684 | - |
dc.identifier.issn | 0950-2688 | - |
dc.identifier.issn | 1469-4409 | - |
dc.identifier.uri | http://hdl.handle.net/2440/114947 | - |
dc.description.abstract | Direct and indirect antigen capture enzyme immunoassays (Ag-EIA) have been developed for the detection of Mycoplasma pneumoniae in nasopharyngeal aspirates or sputum from respiratory infection. The sensitivity of the two Ag-EIA were similar, but the indirect method using polyclonal rabbit and guinea-pig antisera was more convenient. The Ag-EIA had a detection limit of 10(4-4.5) colony-forming units/ml of sample. It was specific for M. pneumoniae and gave a low level response with M. genitalium. There were no cross-reactions with 10 other species of mycoplasmas. Tests with a wide range of bacteria and chlamydia group antigen, representing agents sometimes found in the respiratory tract, were also negative. At the current level of development, the Ag-EIA detected about 90% of specimens that were also positive for culture; 43% of specimens from culture-negative--seropositive patients gave a positive result. The overall pattern of results indicated that while antigen detection is a quick and effective substitute for the slow culture method, serological examination for specific IgM antibody is also necessary to give a complete diagnostic coverage. | - |
dc.description.statementofresponsibility | T.-W. Kok, G. Varkanis, B. P. Marmion, J. Martin and A. Esterman | - |
dc.language.iso | en | - |
dc.publisher | Cambridge University Press | - |
dc.rights | © Cambridge University Press 1988 | - |
dc.source.uri | http://dx.doi.org/10.1017/s0950268800029551 | - |
dc.subject | Nasopharynx | - |
dc.subject | Mycoplasma pneumoniae | - |
dc.subject | Antigens, Bacterial | - |
dc.title | Laboratory diagnosis of Mycoplasma pneumoniae infection: 1. Direct detection of antigen in respiratory exudates by enzyme immunoassay | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1017/S0950268800029551 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Esterman, A. [0000-0001-7324-9171] | - |
Appears in Collections: | Aurora harvest 8 Medicine publications |
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