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https://hdl.handle.net/2440/116102
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Type: | Journal article |
Title: | Random non-coding fragments of lizard DNA: anonymous nuclear loci for the Australian skink, Tiliqua rugosa, and their utility in other Egernia-group species |
Author: | Ansari, T. Bertozzi, T. Hacking, J. Cooper, S. Gardner, M. |
Citation: | Australian Journal of Zoology, 2014; 62(6):515-518 |
Publisher: | CSIRO Publishing |
Issue Date: | 2014 |
ISSN: | 0004-959X 1446-5698 |
Statement of Responsibility: | Talat Hojat Ansari, Terry Bertozzi, Jessica Hacking, Steven J. B. Cooper and Michael G. Gardner |
Abstract: | We report the development of 48 anonymous nuclear loci from the Australian skink Tiliqua rugosa using 454 sequencing. These loci amplified across a Western Australian lineage (47 loci), a ‘northern’ lineage (48 loci) and a ‘southern’ lineage (46 loci). We further tested amplification for the related T. adelaidensis and Egernia stokesii where 37 and 34 loci amplified respectively. The loci showed variability within T. rugosa (22 polymorphic loci) and at least 27 loci also exhibited variation among the three species, highlighting the usefulness of these markers for phylogenetic, phylogeographic and population genetic analyses in T. rugosa and related species. |
Keywords: | DNA markers; phylogeography; squamate |
Rights: | Journal compilation © CSIRO 2014 |
DOI: | 10.1071/ZO14085 |
Published version: | http://dx.doi.org/10.1071/zo14085 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 3 |
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