Adelaide Research & Scholarship
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https://hdl.handle.net/2440/11616
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| Type: | Journal article |
| Title: | Enhanced amplification of hepatitis C virus (HCV) cDNA by PCR: Detection of HCV RNA in archival sera |
| Author: | Beardsley, A. Gowans, E. Burrell, C. Marmion, B. |
| Citation: | Journal of Clinical Microbiology, 1996; 34(6):1581-1582 |
| Publisher: | AMER SOC MICROBIOLOGY |
| Issue Date: | 1996 |
| ISSN: | 0095-1137 1098-660X |
| Statement of Responsibility: | A M Beardsley, E J Gowans, C J Burrell and B P Marmion |
| Abstract: | A reverse transcription-PCR assay which successfully amplified hepatitis C virus RNA from poorly stored archival sera was optimized. Maximum sensitivity was achieved with Moloney murine leukemia virus RNase H- reverse transcriptase and by a single round of PCR amplification of a short (112-bp) fragment of the 5' untranslated region of the viral genome. |
| Keywords: | Humans Hepacivirus Moloney murine leukemia virus Viremia Hepatitis C RNA-Directed DNA Polymerase DNA, Complementary RNA, Viral Sensitivity and Specificity Polymerase Chain Reaction Virology Base Sequence Evaluation Studies as Topic |
| DOI: | 10.1128/jcm.34.6.1581-1582.1996 |
| Published version: | http://dx.doi.org/10.1128/jcm.34.6.1581-1582.1996 |
| Appears in Collections: | Aurora harvest 7 Microbiology and Immunology publications |
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