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https://hdl.handle.net/2440/117525
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Type: | Journal article |
Title: | Primary cilium is required for the stimulating effect of icaritin on osteogenic differentiation and mineralization of osteoblasts in vitro |
Author: | Ma, X.N. Ma, C.X. Shi, W.G. Zhou, J. Ma, H.P. Gao, Y.H. Xian, C.J. Chen, K.M. |
Citation: | Journal of Endocrinological Investigation, 2017; 40(4):357-366 |
Publisher: | Springer |
Issue Date: | 2017 |
ISSN: | 0391-4097 1720-8386 |
Statement of Responsibility: | X.-N. Ma, C.-X. Ma, W.-G. Shi, J. Zhou, H.-P. Ma, Y.-H. Gao, C. J. Xian, K.-M. Chen |
Abstract: | Objective: Icaritin, one effective metabolite of Herba Epimedii-derived flavonoid icariin, has a strong osteogenic activity. However, its action mechanism remains unclear. Since primary cilia have been shown to play a pivotal role in regulating the osteogenesis, we hypothesized primary cilia are indispensable in mediating icaritin osteogenic effect. Materials and methods: Primary rat calvarial osteoblasts were transfected with siRNA1 targeting intraflagellar transport protein 88 (IFT88), a protein required for ciliogenesis, to prevent formation of primary cilium and were treated with 10-6 M icaritin. Results: Alkaline phosphatase (ALP) activity was significantly increased after 3 days in cells transfected with scrambled siRNA control and treated by icaritin (SC+I group) compared to cells transfected with scrambled siRNA control only (SC group). ALP activity after IFT88 siRNA1 transfection and icaritin treatment (siRNA1+I group) was significantly lower than that of SC+I group. Formation of ALP positively stained colonies after 6 days, osteocalcin secretion after 9 days and formation of calcified nodules after 12 days displayed a similar tendency among the three groups. mRNA expression of osteogenesis-related genes ALP, BMP-2, COL1alpha, RUNX-2 and OSX after 24 h was significantly increased in SC+I group, but was not different with SC group in siRNA1+I group. Protein levels of BMP-2, COL1alpha, RUNX-2 and OSX after 48 h showed the similar tendency with gene expression. Conclusion: Primary cilia are important in mediating icaritin-stimulated osteogenic differentiation and may be a novel target for pharmacological therapies for bone loss. |
Keywords: | Primary cilia; icaritin; osteoblasts; small interfering RNA; differentiation; mineralization |
Rights: | © Italian Society of Endocrinology (SIE) 2016. |
DOI: | 10.1007/s40618-016-0568-8 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1042105 |
Published version: | http://dx.doi.org/10.1007/s40618-016-0568-8 |
Appears in Collections: | Aurora harvest 3 Medicine publications |
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