Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/122719
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Type: | Journal article |
Title: | Uterine B cells exhibit regulatory properties during the peri-implantation stage of murine pregnancy |
Author: | Guzman-Genuino, R.M. Eldi, P. Garcia-Valtanen, P. Hayball, J.D. Diener, K.R. |
Citation: | Frontiers in Immunology, 2019; 10:2899-1-2899-12 |
Publisher: | Frontiers Media |
Issue Date: | 2019 |
ISSN: | 1664-3224 1664-3224 |
Statement of Responsibility: | Ruth Marian Guzman-Genuino, Preethi Eldi, Pablo Garcia-Valtanen, John D. Hayball and Kerrilyn R. Diener |
Abstract: | A successful outcome to pregnancy is dependent on the ability of the maternal uterine microenvironment to regulate inflammation processes and establish maternal tolerance. Recently, B cells have been shown to influence pregnancy outcomes as aberrations in their numbers and functions are associated with obstetric complications. In this study, we aimed to comprehensively examine the population frequency and phenotypic profile of B cells over the course of murine pregnancy. Our results demonstrated a significant expansion in B cells within the uterus during the peri-implantation period, accompanied by alterations in B cell phenotype. Functional evaluation of uterine B cells purified from pregnant mice at day 5.5 post-coitus established their regulatory capacity as evidenced by effective suppression of proliferation and activation of syngeneic CD4+ T cells. Flow cytometric analysis revealed that the uterine B cell population has an expanded pool of IL-10-producing B cells bearing upregulated expression of co-stimulatory molecules CD80 and CD86 and activation marker CD27. Our investigations herein demonstrate that during the critical stages surrounding implantation, uterine B cells are amplified and phenotypically modified to act in a regulatory manner that potentially contributes toward the establishment of maternal immunological tolerance in early pregnancy. |
Keywords: | uterine B cells; regulatory B cells; implantation; early pregnancy; suppression |
Rights: | © 2019 Guzman-Genuino, Eldi, Garcia-Valtanen, Hayball and Diener. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
DOI: | 10.3389/fimmu.2019.02899 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1020984 http://purl.org/au-research/grants/nhmrc/1012386 |
Published version: | http://dx.doi.org/10.3389/fimmu.2019.02899 |
Appears in Collections: | Aurora harvest 8 Medicine publications |
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hdl_122719.pdf | Published version | 2.78 MB | Adobe PDF | View/Open |
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