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https://hdl.handle.net/2440/132876
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Type: | Journal article |
Title: | Lipidomic profiling of clinical prostate cancer reveals targetable alterations in membrane lipid composition |
Author: | Butler, L.M. Mah, C.Y. Machiels, J. Vincent, A.D. Irani, S. Mutuku, S. Spotbeen, X. Bagadi, M. Waltregny, D. Moldovan, M. Dehairs, J. Vanderhoydonc, F. Bloch, K. Das, R. Stahl, J. Kench, J.G. Gevaert, T. Derua, R. Waelkens, E. Nassar, Z.D. et al. |
Citation: | Cancer Research, 2021; 81(19):4981-4993 |
Publisher: | American Association for Cancer Research |
Issue Date: | 2021 |
ISSN: | 0008-5472 1538-7445 |
Statement of Responsibility: | Lisa M. Butler, Chui Yan Mah, Jelle Machiels, Andrew D. Vincent, Swati Irani, Shadrack M. Mutuku, Xander Spotbeen, Muralidhararao Bagadi, David Waltregny, Max Moldovan, Jonas Dehairs, Frank Vanderhoydonc, Katarzyna Bloch, Rajdeep Das, Jurgen Stahl, James G. Kench, Thomas Gevaert, Rita Derua, Etienne Waelkens, Zeyad D. Nassar, Luke A. Selth, Paul J. Trim, Marten F. Snel, David J. Lynn, Wayne D. Tilley, Lisa G. Horvath, Margaret M. Centenera, and Johannes V. Swinnen |
Abstract: | Dysregulated lipid metabolism is a prominent feature of prostate cancer that is driven by androgen receptor (AR) signaling. Here we used quantitative mass spectrometry to define the “lipidome” in prostate tumors with matched benign tissues (n = 21), independent unmatched tissues (n = 47), and primary prostate explants cultured with the clinical AR antagonist enzalutamide (n = 43). Significant differences in lipid composition were detected and spatially visualized in tumors compared with matched benign samples. Notably, tumors featured higher proportions of monounsaturated lipids overall and elongated fatty acid chains in phosphatidylinositol and phosphatidylserine lipids. Significant associations between lipid profile and malignancy were validated in unmatched samples, and phospholipid composition was characteristically altered in patient tissues that responded to AR inhibition. Importantly, targeting tumor-related lipid features via inhibition of acetyl-CoA carboxylase 1 significantly reduced cellular proliferation and induced apoptosis in tissue explants. This characterization of the prostate cancer lipidome in clinical tissues reveals enhanced fatty acid synthesis, elongation, and desaturation as tumor-defining features, with potential for therapeutic targeting. Significance: This study identifies malignancy and treatment-associated changes in lipid composition of clinical prostate cancer tissues, suggesting that mediators of these lipidomic changes could be targeted using existing metabolic agents. |
Keywords: | Humans Prostatic Neoplasms Membrane Lipids Neoplasm Staging Spectrometry, Mass, Electrospray Ionization Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Computational Biology Energy Metabolism Male Lipid Metabolism Tandem Mass Spectrometry Metabolomics Molecular Targeted Therapy Neoplasm Grading Biomarkers Transcriptional Regulator ERG Lipidomics |
Rights: | © 2021 American Association for Cancer Research |
DOI: | 10.1158/0008-5472.CAN-20-3863 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1121057 http://purl.org/au-research/grants/nhmrc/1130077 http://purl.org/au-research/grants/nhmrc/1138242 http://purl.org/au-research/grants/nhmrc/1145777 http://purl.org/au-research/grants/arc/FT130101004 http://purl.org/au-research/grants/nhmrc/1138648 http://purl.org/au-research/grants/nhmrc/1196225 |
Published version: | http://dx.doi.org/10.1158/0008-5472.can-20-3863 |
Appears in Collections: | Medicine publications |
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