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https://hdl.handle.net/2440/134792
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Type: | Journal article |
Title: | Implications of Quenching-to-Dequenching Switch in Quantitative Cell Uptake and Biodistribution of Dye-Labeled Nanoparticles |
Author: | Yang, G. Liu, Y. Hui, Y. Tengjisi, Chen, D. Weitz, D.A. Zhao, C.X. |
Citation: | Angewandte Chemie International Edition, 2021; 60(28):15426-15435 |
Publisher: | Wiley |
Issue Date: | 2021 |
ISSN: | 1433-7851 1521-3773 |
Statement of Responsibility: | Guangze Yang, Yun Liu, Yue Hui, Tengjisi, Dong Chen, David A. Weitz, Chun-Xia Zhao |
Abstract: | A general strategy to carry out cell uptake and biodistribution studies is to label nanoparticles (NPs) with a fluorescent dye. However, the comparative study of different dye-loaded NPs remains difficult owing to uncontrolled dye quenching and de-quenching. Here we compared two types of dye-labeled NPs and demonstrated their distinct properties. NPs with dye molecules at a solid state suffer from dye quenching, so the dye release and/or NP degradation in biological environments leads to a several-fold increase of fluorescence intensity despite the same amount of NPs, owing to the state switch from quenching to de-quenching. In contrast, NPs with dye molecules at a soluble state exhibit no quenching effect. To standardize the comparative study, we propose two possible solutions: using lower dye loading or using medium analysis for quantifying cell uptake of NPs. This work provides valuable insights into selecting valid quantification methods for bio-nano studies. |
Keywords: | biodistribution; cell uptake; fluorescence; nanoparticles; quenching |
Rights: | © 2021 Wiley-VCH GmbH |
DOI: | 10.1002/anie.202101730 |
Grant ID: | http://purl.org/au-research/grants/arc/FT140100726 http://purl.org/au-research/grants/arc/DP200101238 |
Published version: | http://dx.doi.org/10.1002/anie.202101730 |
Appears in Collections: | Chemical Engineering publications |
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