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https://hdl.handle.net/2440/13481
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DC Field | Value | Language |
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dc.contributor.author | Kaku, Hanae | en |
dc.contributor.author | Shibuya, Naoto | en |
dc.contributor.author | Xu, P. | en |
dc.contributor.author | Aryan, Arun Prakash | en |
dc.contributor.author | Fincher, Geoffrey B. | en |
dc.date.issued | 1997 | en |
dc.identifier.citation | Physiologia Planatarum, 1997; 100(1):111-118 | en |
dc.identifier.issn | 0031-9317 | en |
dc.identifier.uri | http://hdl.handle.net/2440/13481 | - |
dc.description | Article first published online: 28 APR 2006 | en |
dc.description.abstract | N-acetylchitooligosaccharides of degree of polymerization 6 to 8 elicit the synthesis of (1→3)-β-glucan endohydrolase (EC 3.2.1.39) activity in suspension-cultured cells derived from immature barley (Hordeum vulgare) embryos. Corresponding de-acety-lated chitooligosaccharides have no effect. Concentrations of N-acetylchitoheptaose in the 200 nM range are sufficient to elicit the response. A 2- to 3-fold increase in (1→3)-β-glucanase activity is detected 24 h after the addition of the oligosaccharide. Non-denaturing gel electrophoresis, coupled with the in situ detection of (1→3)-β-glucanase activity in the gels, has enabled the separation of specific isoforms of the enzyme. The increase in (1→3)-β-glucanase activity following N-acetylchitoheptaose induction is attributable predominantly to enhanced levels of isoenzyme GII, although the barley (1→3)-β-glucanase gene family encodes at least seven different isoforms of the enzyme. Northern hybridization analyses with gene-specific probes confirmed the presence of mRNA encoding isoenzyme GII as the major mRNA in cells treated with the oligosaccharide elicitor. The results therefore demonstrate a specific induction of the (1→3)-β-glucanase isoenzyme GII gene following stimulation of barley cells with oligosaccharides of fungal cell wall origin, and further suggest that a plant's response to microbial attack involves transcription of quite specific members of the gene families that encode pathogenesis-related proteins. | en |
dc.description.statementofresponsibility | Hanae Kaku, Naoto Shibuya, Peilin Xu, Arun P. Aryan and Geoffrey B. Fincher | en |
dc.language.iso | en | en |
dc.rights | Copyright © Physiologia Plantarum | en |
dc.subject | Barley; cell cultures; chitin; elicitors; fungal pathogens; plant-pathogen interactions | en |
dc.title | N-acetylchitooligosaccharides elicit expression of a single (1→3)-β-glucanase gene in suspension-cultured from barley (Hordeum vulgare) | en |
dc.title.alternative | N-acetylchitooligosaccharides elicit expression of a single (1->3)-beta-glucanase gene in suspension-cultured from barley (Hordeum vulgare) | en |
dc.type | Journal article | en |
dc.identifier.doi | 10.1111/j.1399-3054.1997.tb03460.x | en |
Appears in Collections: | Agriculture, Food and Wine publications |
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