Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/137279
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Label-Free Isolation and Single Cell Biophysical Phenotyping Analysis of Primary Cardiomyocytes Using Inertial Microfluidics. |
Author: | Tavassoli, H. Rorimpandey, P. Kang, Y.C. Carnell, M. Brownlee, C. Pimanda, J.E. Chan, P.P.Y. Chandrakanthan, V. |
Citation: | Small, 2021; 17(8) |
Publisher: | Wiley |
Issue Date: | 2021 |
ISSN: | 1613-6810 1613-6829 |
Statement of Responsibility: | Hossein Tavassoli, Prunella Rorimpandey, Young Chan Kang, Michael Carnell, Chris Brownlee, John E Pimanda, Peggy P.Y. Chan, and Vashe Chandrakanthan |
Abstract: | To advance the understanding of cardiomyocyte (CM) identity and function, appropriate tools to isolate pure primary CMs are needed. A label-free method to purify viable CMs from mouse neonatal hearts is developed using a simple particle size-based inertial microfluidics biochip achieving purities of over 90%. Purified CMs are viable and retained their identity and function as depicted by the expression of cardiac-specific markers and contractility. The physico-mechanical properties of sorted cells are evaluated using downstream real-time deformability cytometry. CMs exhibited different physico-mechanical properties when compared with non-CMs. Taken together, this CM isolation and phenotyping method could serve as a valuable tool to progress the understanding of CM identity and function, and ultimately benefit cell therapy and diagnostic applications. |
Keywords: | cardiomyocyte; cell separation; deformability cytometry; microfluidics; regeneration |
Rights: | © 2020 Wiley-VCH GmbH |
DOI: | 10.1002/smll.202006176 |
Grant ID: | http://purl.org/au-research/grants/arc/DP170103704 |
Published version: | http://dx.doi.org/10.1002/smll.202006176 |
Appears in Collections: | Medicine publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.