Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/137961
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Identification of new potential downstream transcriptional targets of the strigolactone pathway including glucosinolate biosynthesis. |
Author: | Hellens, A.M. Chabikwa, T.G. Fichtner, F. Brewer, P.B. Beveridge, C.A. |
Citation: | Plant Direct, 2023; 7(3):e486-e486 |
Publisher: | Wiley |
Issue Date: | 2023 |
ISSN: | 2475-4455 2475-4455 |
Statement of Responsibility: | Alicia M. Hellens, Tinashe G. Chabikwa, Franziska Fichtner, Philip B. Brewer, Christine A. Beveridge |
Abstract: | Strigolactones regulate shoot branching and many aspects of plant growth, development, and allelopathy. Strigolactones are often discussed alongside auxin because they work together to inhibit shoot branching. However, the roles and mechanisms of strigolactones and how they act independently of auxin are still elusive. Additionally, there is still much in general to be discovered about the network of molecular regulators and their interactions in response to strigolactones. Here, we conducted an experiment in Arabidopsis with physiological treatments and strigolactone mutants to determine transcriptional pathways associated with strigolactones. The three physiological treatments included shoot tip removal with and without auxin treatment and treatment of intact plants with the auxin transport inhibitor, N-1-naphthylphthalamic acid (NPA). We identified the glucosinolate biosynthesis pathway as being upregulated across strigolactone mutants indicating strigolactone-glucosinolate crosstalk. Additionally, strigolactone application cannot restore the highly branched phenotype observed in glucosinolate biosynthesis mutants, placing glucosinolate biosynthesis downstream of strigolactone biosynthesis. Oxidative stress genes were enriched across the experiment suggesting that this process is mediated through multiple hormones. Here, we also provide evidence supporting non-auxin-mediated, negative feedback on strigolactone biosynthesis. Increases in strigolactone biosynthesis gene expression seen in strigolactone mutants could not be fully restored by auxin. By contrast, auxin could fully restore auxin-responsive gene expression increases, but not sugar signaling-related gene expression. Our data also point to alternative roles of the strigolactone biosynthesis genes and potential new signaling functions of strigolactone precursors. In this study, we identify a strigolactone-specific regulation of glucosinolate biosynthesis genes indicating that the two are linked and may work together in regulating stress and shoot ranching responses in Arabidopsis. Additionally, we provide evidence for non-auxinmediated feedback on strigolactone biosynthesis and discuss this in the context of sugar signaling. |
Keywords: | Arabidopsis thaliana auxin flavonoids glucosinolates strigolactones transcriptomic |
Rights: | © 2023 The Authors. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
DOI: | 10.1002/pld3.486 |
Grant ID: | http://purl.org/au-research/grants/arc/CE200100015 http://purl.org/au-research/grants/arc/FT180100081 |
Published version: | http://dx.doi.org/10.1002/pld3.486 |
Appears in Collections: | Agriculture, Food and Wine publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
hdl_137961.pdf | Published version | 6.55 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.