Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/140358
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Type: | Journal article |
Title: | Quantifying EGFR endosomal recycling via immunofluorescence in breast cancer cells |
Author: | Lonic, A. Onglao, W. Khew-Goodall, Y. |
Citation: | STAR Protocols, 2022; 3(2):101305-1-101305-12 |
Publisher: | Elsevier BV |
Issue Date: | 2022 |
ISSN: | 2666-1667 2666-1667 |
Statement of Responsibility: | Ana Lonic, Winona Onglao, and Yeesim Khew-Goodall |
Abstract: | Previously published protocols for quantification of endosomal recycling are limited by the use of radioactive reagents, washing of cells in reducing buffers, or the requirement for large numbers of cells. Here, we describe a protocol for quantification of endosomal recycling using immunofluorescence that is optimized for EGFR in BT-549 breast cancer cells but could be applied to other RTKs and cell lines. Our protocol enables quick assessment of recycling and uses a relatively small number of cells. |
Keywords: | Cell Biology; Cancer; Microscopy; Signal Transduction |
Rights: | © 2022 The Authors. This article is available under the Creative Commons CC-BY-NC-ND license and permits non-commercial use of the work as published, without adaptation or alteration provided the work is fully attributed. |
DOI: | 10.1016/j.xpro.2022.101305 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1146087 |
Published version: | http://dx.doi.org/10.1016/j.xpro.2022.101305 |
Appears in Collections: | Medicine publications |
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hdl_140358.pdf | Published version | 2.1 MB | Adobe PDF | View/Open |
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