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https://hdl.handle.net/2440/16995
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Type: | Journal article |
Title: | A NAC domain protein interacts with tomato leaf curl virus replication accessory protein and enhances viral replication |
Author: | Selth, L. Dogra, S. Rasheed, M. Healy, H. Randles, J. Rezaian, M. |
Citation: | The Plant Cell, 2005; 17(1):311-325 |
Publisher: | Amer Soc Plant Physiologists |
Issue Date: | 2005 |
ISSN: | 1040-4651 1532-298X |
Statement of Responsibility: | Luke A. Selth, Satish C. Dogra, M. Saif Rasheed, Helen Healy, John W. Randles and M. Ali Rezaian |
Abstract: | Geminivirus replication enhancer (REn) proteins dramatically increase the accumulation of viral DNA species by an unknown mechanism. In this study, we present evidence implicating SlNAC1, a new member of the NAC domain protein family from tomato (Solanum lycopersicum), in Tomato leaf curl virus (TLCV) REn function. We isolated SlNAC1 using yeast (Saccharomyces cerevisiae) two-hybrid technology and TLCV REn as bait, and confirmed the interaction between these proteins in vitro. TLCV induces SlNAC1 expression specifically in infected cells, and this upregulation requires REn. In a transient TLCV replication system, overexpression of SlNAC1 resulted in a substantial increase in viral DNA accumulation. SlNAC1 colocalized with REn to the nucleus and activated transcription of a reporter gene in yeast, suggesting that in healthy cells it functions as a transcription factor. Together, these results imply that SlNAC1 plays an important role in the process by which REn enhances TLCV replication. |
Keywords: | Cell Nucleus Plant Viruses Plant Proteins Transcription Factors Viral Proteins DNA, Viral Evolution, Molecular Phylogeny Virus Replication Plant Diseases Gene Expression Regulation, Plant Gene Expression Regulation, Viral Up-Regulation Amino Acid Sequence Base Sequence Protein Structure, Tertiary Molecular Sequence Data Transcriptional Activation Solanum lycopersicum |
Description: | © 2004 American Society of Plant Biologists |
DOI: | 10.1105/tpc.104.027235 |
Published version: | http://dx.doi.org/10.1105/tpc.104.027235 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 6 |
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