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https://hdl.handle.net/2440/23834
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dc.contributor.author | Burton, R. | - |
dc.contributor.author | Wilson, S. | - |
dc.contributor.author | Hrmova, M. | - |
dc.contributor.author | Harvey, A. | - |
dc.contributor.author | Shirley, N. | - |
dc.contributor.author | Medhurst, A. | - |
dc.contributor.author | Stone, B. | - |
dc.contributor.author | Newbigin, E. | - |
dc.contributor.author | Bacic, A. | - |
dc.contributor.author | Fincher, G. | - |
dc.date.issued | 2006 | - |
dc.identifier.citation | Science, 2006; 311(5769):1940-1942 | - |
dc.identifier.issn | 0036-8075 | - |
dc.identifier.issn | 1095-9203 | - |
dc.identifier.uri | http://hdl.handle.net/2440/23834 | - |
dc.description.abstract | A characteristic feature of grasses and commercially important cereals is the presence of (1,3;1,4)-ß-D-glucans in their cell walls. We have used comparative genomics to link a major quantitative trait locus for (1,3;1,4)-ß-D-glucan content in barley grain to a cluster of cellulose synthase–like CslF genes in rice. After insertion of rice CslF genes into Arabidopsis, we detected (1,3;1,4)-ß-D-glucan in walls of transgenic plants using specific monoclonal antibodies and enzymatic analysis. Because wild-type Arabidopsis does not contain CslF genes or have (1,3;1,4)-ß-D-glucans in its walls, these experiments provide direct, gain-of-function evidence for the participation of rice CslF genes in (1,3;1,4)-ß-D-glucan biosynthesis. | - |
dc.description.statementofresponsibility | Rachel A. Burton, Sarah M. Wilson, Maria Hrmova, Andrew J. Harvey, Neil J. Shirley, Anne Medhurst, Bruce A. Stone, Edward J. Newbigin, Antony Bacic, Geoffrey B. Fincher | - |
dc.language.iso | en | - |
dc.publisher | Amer Assoc Advancement Science | - |
dc.source.uri | http://dx.doi.org/10.1126/science.1122975 | - |
dc.subject | Cell Wall | - |
dc.subject | Plants, Genetically Modified | - |
dc.subject | Arabidopsis | - |
dc.subject | Hordeum | - |
dc.subject | Plant Leaves | - |
dc.subject | Glucosyltransferases | - |
dc.subject | beta-Glucans | - |
dc.subject | Antibodies, Monoclonal | - |
dc.subject | Genomics | - |
dc.subject | Transformation, Genetic | - |
dc.subject | Synteny | - |
dc.subject | Genes, Plant | - |
dc.subject | Genome, Plant | - |
dc.subject | Quantitative Trait Loci | - |
dc.subject | Oryza | - |
dc.title | Cellulose synthase-like CslF genes mediate the synthesis of cell wall (1,3;1,4)-beta-D-glucans | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1126/science.1122975 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Burton, R. [0000-0002-0638-4709] | - |
dc.identifier.orcid | Hrmova, M. [0000-0002-3545-0605] | - |
dc.identifier.orcid | Shirley, N. [0000-0001-8114-9891] | - |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 2 |
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