Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/28134
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Analysis of Drosophila Cyclin EI and II function during development: Identification of an inhibitory zone within the morphogenetic furrow of the eye imaginal disc that blocks the function of Cyclin EI but not Cyclin EII |
Author: | Denton, D. Secombe, J. Coombe, M. Brumby, A. Saint, R. Richardson, H. |
Citation: | Developmental Biology, 2002; 241(1):157-171 |
Publisher: | Academic Press Inc |
Issue Date: | 2002 |
ISSN: | 0012-1606 1095-564X |
Organisation: | Centre for the Molecular Genetics of Development |
Abstract: | The Drosophilacyclin E (DmcycE) gene gives rise to two transcripts encoding proteins that differ at their N termini, DmcycEII and DmcycEI. This study presents the first in vivo dissection of Cyclin E function. Ectopic expression studies using N- and C-terminal deletions of DmcycEI revealed that a region of 322 residues surrounding the cyclin box is sufficient to induce entry of G₁-arrested larval eye imaginal disc cells into S phase. Ectopic expression of DmcycEI in the eye disc has been previously shown to drive anterior, but not posterior, G₁-phase cells within the morphogenetic furrow (MF) into S phase. Significantly, ectopic expression of DmcycEII and N-terminal deletions of DmcycEI were able to drive all G₁ cells within the morphogenetic furrow into S phase, while a C-terminal deletion of DmcycEI could not. The p21 homolog Dacapo was shown by yeast two-hybrid, coimmunolocalization, and in vivo functional studies not to be the mediator of the DmcycEI inhibition in posterior part of the MF. Taken together, these results reveal a novel zone within the posterior region of the MF where DmcycEI but not DmcycEII function is inhibited, and suggest that DmcycEII is a more potent inducer of S phase. |
Keywords: | Cyclin E Dacapo G1-S phase cell cycle Drosophila development |
DOI: | 10.1006/dbio.2001.0496 |
Description (link): | http://www.sciencedirect.com/science/journal/00121606 |
Published version: | http://dx.doi.org/10.1006/dbio.2001.0496 |
Appears in Collections: | Aurora harvest 2 Centre for the Molecular Genetics of Development publications Molecular and Biomedical Science publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.