Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/38979
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dc.contributor.authorRoffey, D.-
dc.contributor.authorLuscombe-Marsh, N.-
dc.contributor.authorByrne, N.-
dc.contributor.authorHills, A.-
dc.contributor.authorBellon, M.-
dc.contributor.authorTsopelas, C.-
dc.contributor.authorKirkwood, I.-
dc.contributor.authorWittert, G.-
dc.date.issued2005-
dc.identifier.citationAsia Pacific Journal of Clinical Nutrition, 2005; 14(1):83-90-
dc.identifier.issn0964-7058-
dc.identifier.issn1440-6047-
dc.identifier.urihttp://hdl.handle.net/2440/38979-
dc.description.abstractThe aim of this study was to evaluate the utility of the [¹⁴C]-sodium bicarbonate/urea technique to detect physical activity-induced increases in total energy expenditure in free-living healthy men. Thirteen healthy males aged 34.1 ± 11.7 yrs with body mass index 24.1 ± 3.1 kg/m2 were studied on three separate occasions, during which [¹⁴C]-bicarbonate was infused over 48-hours and urine was collected during the second 24-hours. On three separate occasions and in random order, subjects either remained sedentary, or performed a bout of physical activity on an electro-magnetically braked cycle ergometer sufficient to increase energy expenditure by 7% or 11% above predicted sedentary total energy expenditure. Urine samples were analyzed to evaluate the amount of [¹⁴C]-bicarbonate incorporated into urinary urea, thereby reflecting the amount of CO₂ produced per day, and upon conversion, the number of kilojoules of energy expended in 24-hours. All 13 subjects successfully completed the two physical activity treatments and there were no adverse events. As measured by the [¹⁴C]-urea assay, mean total energy expenditure values were not significantly different between sedentary activity (17902 ± 905 kJ/day), the physical activity treatment designed to increase TEE by 7% (17701 ± 594 kJ/day) and the physical activity treatment designed to increase TEE by 11% (18538 ± 485 kJ/day) (P=0.668). In conclusion, although the [¹⁴C]-sodium bicarbonate/urea technique was well tolerated and did not interfere with normal daily activities, it was not able to accurately measure physical activity-induced increases in EE in the range of 7-11% above predicted sedentary total energy expenditure.-
dc.description.statementofresponsibilityDarren M Roffey, Natalie D Luscombe, Nuala M Byrne, Andrew P Hills, Max Bellon, Chris Tsopelas, Ian D Kirkwood and Gary A Wittert-
dc.language.isoen-
dc.publisherH E C Press-
dc.rightsCopyright © 2001-2005 Healthy Eating Club-
dc.source.urihttp://www.healthyeatingclub.org/APJCN/Volume14/vol14.1/fullArticles/Roffey.pdf-
dc.subjectenergy expenditure-
dc.subjectcarbon dioxide production-
dc.subjectexercise prescription-
dc.subjectsodium bicarbonate-
dc.subjecturea-
dc.titleUse of [¹⁴C]-sodium bicarbonate/urea to measure physical activity induced increases in total energy expenditure in free-living healthy males-
dc.title.alternativeUse of [(14)C]-sodium bicarbonate/urea to measure physical activity induced increases in total energy expenditure in free-living healthy males-
dc.typeJournal article-
pubs.publication-statusPublished-
dc.identifier.orcidLuscombe-Marsh, N. [0000-0001-9690-4722]-
dc.identifier.orcidWittert, G. [0000-0001-6818-6065]-
Appears in Collections:Aurora harvest
Medicine publications

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