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https://hdl.handle.net/2440/44317
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Type: | Journal article |
Title: | Genomic regions of tomato leaf curl virus DNA satellite required for replication and for satellite-mediated delivery of heterologous DNAs |
Author: | Li, D. Akbar Behjatnia, S. Dry, I. Randles, J. Eini Gandomani, O. Rezaian, M. |
Citation: | Journal of General Virology, 2007; 88(7):2073-2077 |
Publisher: | Soc General Microbiology |
Issue Date: | 2007 |
ISSN: | 0022-1317 1465-2099 |
Statement of Responsibility: | Dongmei Li, S. A. Akbar Behjatnia, Ian B. Dry, John W. Randles, Omid Eini and M. Ali Rezaian |
Abstract: | Tomato leaf curl virus (TLCV) satellite DNA (sat-DNA) is a 682 nt, circular, single-stranded molecule that lacks an open reading frame (ORF) or an apparent promoter. It contains binding motifs for the TLCV replication-associated protein, but these are dispensable for replication. To identify the regions of the sat-DNA critical for replication, the entire sequence was scanned by deletion/replacement mutagenesis. Transient assays using Nicotiana benthamiana revealed that sequences within nt 296–35 (through nt 682) are essential for replication. Sequence deletions and replacements between nt 35 and 296 were tolerated but with a significant loss of infectivity, indicating that genome size strongly influences replication efficiency. Within the permissible region, inserts of 100–700 nt were retained in transient assays although with a slight reduction in replication. In addition, sat-DNA constructs containing short non-viral DNAs replicated and spread in tobacco plants, indicating their potential as gene-delivery vectors. |
Keywords: | Plants, Genetically Modified Tobacco Glucuronidase DNA, Recombinant DNA, Satellite DNA, Viral Virus Replication Plant Diseases Gene Silencing Mutagenesis Sequence Deletion Base Sequence Genes, Reporter Genome, Viral Genetic Vectors Begomovirus Solanum lycopersicum |
Description: | Copyright © 2007 by the Society for General Microbiology. |
DOI: | 10.1099/vir.0.82853-0 |
Published version: | http://vir.sgmjournals.org/cgi/content/abstract/88/7/2073 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest |
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