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https://hdl.handle.net/2440/46417
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Type: | Journal article |
Title: | A novel tetrafunctional reagent for simultaneous intramolecular and intermolecular cross-linking of bovine hemoglobin |
Author: | Yun, Q. He, M. Xing, W. Bi, J. Ma, G. Su, Z. |
Citation: | Biotechnology Letters, 2004; 26(17):1359-1363 |
Publisher: | Kluwer Academic Publ |
Issue Date: | 2004 |
ISSN: | 0141-5492 1573-6776 |
Statement of Responsibility: | Qiang Yun, Minglei He, Wenchao Xing, Jingxiu Bi, Guanghui Ma & Zhiguo Su |
Abstract: | A novel tetrafunctional reagent, α, γ, α, γ-tetra-succinimidyl-hexanediamide-di-glutamate ester (HDG(OSu)4), was successfully synthesized, and a well-defined cross-linked bovine hemoglobin (mainly 128 kDa) was prepared with this reagent. Due to the spatial structure of this cross-linking reagent, the intramolecular and intermolecular cross-linking of bovine hemoglobin was formed simultaneously in one reaction. Although the cross-linked bovine hemoglobin showed a slight decrease in half-saturated O2 pressure value (P50, from 28.1 mm Hg to 21.7 mm Hg) and Hill coefficient (from 2.5 to 2), due to the cross-linkage, it still performed well for O2 delivery. |
Keywords: | blood substitute bovine hemoglobin cross-linking reagent modification |
Description: | The definitive version may be found at http://www.springerlink.com/ |
DOI: | 10.1023/B:BILE.0000045633.36744.0d |
Published version: | http://www.springerlink.com/content/n82181w362282106/ |
Appears in Collections: | Aurora harvest 6 Chemical Engineering publications |
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