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https://hdl.handle.net/2440/46425
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Type: | Journal article |
Title: | Polyethylene glycol improves conjugation of bovine hemoglobin and human serum albumin in a controlled ratio |
Author: | Zheng, C. Bi, J. Ma, G. Su, Z. |
Citation: | Artificial Cells Blood Substitutes and Biotechnology, 2007; 35(6):568-584 |
Publisher: | Marcel Dekker Inc |
Issue Date: | 2007 |
ISSN: | 1073-1199 1532-4184 |
Statement of Responsibility: | Chunyang Zheng, Jingxiu Bi, Guanghui Ma, Zhiguo Su |
Abstract: | Direct conjugation of bovine hemoglobin (Hb) and human serum albumin (HSA) with glutaraldehyde would result in a complex mixture of dimers Hb-Hb, Hb-HSA, HSA-HSA and other oligomers. To obtain a high yield of target Hb-HSA, modulation of the reaction environment was carried out. It was found that polyethylene glycol (PEG), a hydrophilic polymer, could improve the yield of Hb-HSA conjugate. The degree of improvement depended on the molecular weight and concentration of PEG. Under optimum condition of 9% (w/v) of PEG 4000, the reaction proceeded in a controlled mode with conversion yield of starting proteins to Hb-HSA increasing from 6% to 30%. The purity was about 88% of the total conjugates. Furthermore, the impurities were mainly tetrameric molecules of two Hb-HSA conjugates. The improvement could be attributed to the “micro-compartment” created by addition of polyethylene glycol, which brings HSA and Hb close together, thus increasing the chance of conjugation between the two molecules. |
Keywords: | Blood substitute Bovine hemoglobin Glutaraldehyde Human serum albumin Polyethylene glycol |
DOI: | 10.1080/10731190701586236 |
Description (link): | http://www.informaworld.com/smpp/content~db=all?content=10.1080/10731190701586236 |
Published version: | http://dx.doi.org/10.1080/10731190701586236 |
Appears in Collections: | Aurora harvest Chemical Engineering publications |
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